• Product name
    Anti-TRAP1 antibody [EPR5381]
    See all TRAP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5381] to TRAP1
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TRAP1 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • WB: K562, HL60, 293T and HeLa cell lysates IHC-P: Human kidney and liver tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/5000.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Chaperone that expresses an ATPase activity.
    • Tissue specificity
      Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
    • Sequence similarities
      Belongs to the heat shock protein 90 family.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Heat shock protein 75 kDa antibody
      • Heat shock protein 75 kDa, mitochondrial antibody
      • HSP 75 antibody
      • HSP75 antibody
      • HSP90L antibody
      • mitochondrial antibody
      • TNF receptor associated protein 1 antibody
      • TNFR-associated protein 1 antibody
      • TRAP-1 antibody
      • Trap1 antibody
      • TRAP1_HUMAN antibody
      • Tumor necrosis factor type 1 receptor-associated protein antibody
      see all


    • All lanes : Anti-TRAP1 antibody [EPR5381] (ab109323) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 80 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?

    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human liver tissue by Immunohistochemistry.


    This product has been referenced in:
    • Gao C  et al. Overexpression of the mitochondrial chaperone tumor necrosis factor receptor-associated protein 1 is associated with the poor prognosis of patients with colorectal cancer. Oncol Lett 15:5451-5458 (2018). Read more (PubMed: 29552185) »
    • Megger DA  et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013). Read more (PubMed: 23462207) »
    See all 2 Publications for this product

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