Anti-TRAP100/MED24 antibody (ab194951)
Key features and details
- Rabbit polyclonal to TRAP100/MED24
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRAP100/MED24 antibody -
Description
Rabbit polyclonal to TRAP100/MED24 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human TRAP100/MED24 aa 800-900. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Synthetic peptide around Lys834 of Human TRAP100/MED24.
Database link: O75448 -
Positive control
- 293 cell extract; Human Brain, Cortex tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 µg/ml.
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WB |
1/500 - 1/1000.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/1000. |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
Tissue specificity
Ubiquitous. Abundant in skeletal muscle, heart and placenta. -
Sequence similarities
Belongs to the Mediator complex subunit 24 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9862 Human
- Entrez Gene: 23989 Mouse
- Entrez Gene: 619436 Rat
- Omim: 607000 Human
- SwissProt: O75448 Human
- SwissProt: Q99K74 Mouse
- SwissProt: Q4V8B3 Rat
- Unigene: 462983 Human
see all -
Alternative names
- CRSP complex subunit 4 antibody
- Activator recruited cofactor 100 kDa component antibody
- Activator-recruited cofactor 100 kDa component antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab194951 has not yet been referenced specifically in any publications.