Product nameAnti-TRAP220/MED1 (phospho T1457) antibody [EPR13269]
See all TRAP220/MED1 primary antibodies
DescriptionRabbit monoclonal [EPR13269] to TRAP220/MED1 (phospho T1457)
Tested applicationsSuitable for: Dot blot, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TRAP220/MED1 aa 1450-1550 (phospho T1457). The exact sequence is proprietary.
Database link: Q15648
- HeLa cell lysate, Human pancreas tissue and Human thyroid gland carcinoma tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol
PurityTissue culture supernatant
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab181103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 168 kDa.|
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the Mediator complex subunit 1 family.
modificationsPhosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
- Information by UniProt
- Activator-recruited cofactor 205 kDa component antibody
- ARC205 antibody
- CRSP1 antibody
All lanes : Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (ab181103) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 168 kDa
Immunohistochemical analysis of paraffin-embedded sections of Human thyroid gland carcinoma tissue labeling TRAP220/MED1 (phospho T1457) using ab181103 at a 1/250 dilution, followed by a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded sections of Human pancreas tissue labeling TRAP220/MED1 (phospho T1457) using ab181103 at a 1/250 dilution, followed by a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
Dot blot analysis of TRAP220/MED1 (pT1457) peptide (Lane 1), TRAP220/MED1 non-phospho peptide (Lane 2) labelling TRAP220/MED1 (pT1457) with ab181103 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
This product has been referenced in:
- Chen Q et al. An Experimental Analysis of the Molecular Effects of Trastuzumab (Herceptin) and Fulvestrant (Falsodex), as Single Agents or in Combination, on Human HR+/HER2+ Breast Cancer Cell Lines and Mouse Tumor Xenografts. PLoS One 12:e0168960 (2017). WB . Read more (PubMed: 28045951) »