Overview

  • Product name

    Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269]
    See all TRAP220/MED1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13269] to TRAP220/MED1 (phospho T1457)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TRAP220/MED1 aa 1450-1550 (phospho T1457). The exact sequence is proprietary.
    Database link: Q15648

  • Positive control

    • HeLa cell lysate, Human pancreas tissue and Human thyroid gland carcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
IHC-P 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 168 kDa.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the Mediator complex subunit 1 family.
  • Post-translational
    modifications

    Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator-recruited cofactor 205 kDa component antibody
    • ARC205 antibody
    • CRSP1 antibody
    • CRSP200 antibody
    • DRIP205 antibody
    • DRIP230 antibody
    • MED1 antibody
    • MED1_HUMAN antibody
    • Mediator complex subunit 1 antibody
    • Mediator of RNA polymerase II transcription subunit 1 antibody
    • p53 regulatory protein RB18A antibody
    • PBP antibody
    • Peroxisome proliferator-activated receptor-binding protein antibody
    • PPAR binding protein antibody
    • PPAR-binding protein antibody
    • PPARBP antibody
    • PPARGBP antibody
    • RB18A antibody
    • Thyroid hormone receptor-associated protein complex 220 kDa component antibody
    • Thyroid receptor-interacting protein 2 antibody
    • TR-interacting protein 2 antibody
    • Trap220 antibody
    • TRIP-2 antibody
    • TRIP2 antibody
    • Vitamin D receptor-interacting protein complex component DRIP205 antibody
    see all

Images

  • All lanes : Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (ab181103) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with Lambda Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 168 kDa

  • Immunohistochemical analysis of paraffin-embedded sections of Human thyroid gland carcinoma tissue labeling TRAP220/MED1 (phospho T1457) using ab181103 at a 1/250 dilution, followed by a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded sections of Human pancreas tissue labeling TRAP220/MED1 (phospho T1457) using ab181103 at a 1/250 dilution, followed by a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

  • Dot blot analysis of TRAP220/MED1 (pT1457) peptide (Lane 1), TRAP220/MED1 non-phospho peptide (Lane 2) labelling TRAP220/MED1 (pT1457) with ab181103 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

This product has been referenced in:

  • Chen Q  et al. An Experimental Analysis of the Molecular Effects of Trastuzumab (Herceptin) and Fulvestrant (Falsodex), as Single Agents or in Combination, on Human HR+/HER2+ Breast Cancer Cell Lines and Mouse Tumor Xenografts. PLoS One 12:e0168960 (2017). WB . Read more (PubMed: 28045951) »
See 1 Publication for this product

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