Key features and details
- Mouse monoclonal [24-31] to TRAP/CD40L (Biotin)
- Suitable for: Flow Cyt
- Reacts with: Human, Cynomolgus monkey, Rhesus monkey
- Conjugation: Biotin
- Isotype: IgG1
Product nameAnti-TRAP/CD40L antibody [24-31] (Biotin)
See all TRAP/CD40L primary antibodies
DescriptionMouse monoclonal [24-31] to TRAP/CD40L (Biotin)
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human, Cynomolgus monkey, Rhesus monkey
Fusion protein corresponding to TRAP/CD40L.
- TPA+ ionomycin-stimulated Human PBMCs
General notesStore undiluted.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
- Anti-TRAP/CD40L antibody [24-31] (FITC) (ab134404)
- Anti-TRAP/CD40L antibody [24-31] (PE) (ab134405)
- Anti-TRAP/CD40L antibody [24-31] (APC) (ab134406)
- Anti-TRAP/CD40L antibody [24-31] - Low endotoxin, Azide free (ab234178)
- Anti-TRAP/CD40L antibody [24-31] (PE) (ab234220)
- Anti-TRAP/CD40L antibody [24-31] (FITC) (ab234271)
Our Abpromise guarantee covers the use of ab134407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 106 cells.
Use 100 µl test volume.
FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
ab134407 has been referenced in 2 publications.
- Matus-Nicodemos R et al. Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells. J Immunol 186:2164-71 (2011). Flow Cyt ; Human . PubMed: 21242519
- Kuchen S et al. Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol 179:5886-96 (2007). Flow Cyt ; Human . PubMed: 17947662