Product nameAnti-TRAP/CD40L antibody [24-31] - Low endotoxin, Azide free
See all TRAP/CD40L primary antibodies
DescriptionMouse monoclonal [24-31] to TRAP/CD40L - Low endotoxin, Azide free
Tested applicationsSuitable for: Functional Studies, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
Fusion protein corresponding to TRAP/CD40L.
- Activated Human peripheral blood leukocytes.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
- Anti-TRAP/CD40L antibody [24-31] (ab134403)
- Anti-TRAP/CD40L antibody [24-31] (FITC) (ab134404)
- Anti-TRAP/CD40L antibody [24-31] (Phycoerythrin) (ab134405)
- Anti-TRAP/CD40L antibody [24-31] (Allophycocyanin) (ab134406)
- Anti-TRAP/CD40L antibody [24-31] (Biotin) (ab134407)
- Anti-TRAP/CD40L antibody [24-31] - Low endotoxin, Azide free (ab234178)
- Anti-TRAP/CD40L antibody [24-31] (Phycoerythrin) (ab234220)
- Anti-TRAP/CD40L antibody [24-31] (FITC) (ab234271)
Our Abpromise guarantee covers the use of ab171235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171235 has been tested by flow cytometric analysis of activated human peripheral blood leukocytes. This can be used at less than or equal to 1μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. ab171235 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
ab171235 has not yet been referenced specifically in any publications.