Anti-TRAP/CD40L antibody (ab2391)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-TRAP/CD40L antibody
See all TRAP/CD40L primary antibodies -
DescriptionRabbit polyclonal to TRAP/CD40L
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, ELISA, WB, IHC-P, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to TRAP/CD40L. surrounding amino acid 246 of human CD40 Ligand
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Positive control
- Human muscle tissue sections
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/200. See Abreview | |
| ELISA | Use a concentration of 0.5 - 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | 1/100. Fix with acetone -20°C for 10 min (provides a better signal than unfixed tissue; PFA 4% abolishes the signal). Incubate primary for 24h at 4°C. Use amplification (indirect IF). |
Target
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FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
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Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
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Sequence similaritiesBelongs to the tumor necrosis factor family.
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Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 959 Human
- Entrez Gene: 21947 Mouse
- Entrez Gene: 84349 Rat
- Omim: 300386 Human
- SwissProt: P29965 Human
- SwissProt: P27548 Mouse
- SwissProt: Q9Z2V2 Rat
- Unigene: 592244 Human
see all -
Alternative names
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
Images
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Flow Cytometry - Anti-TRAP/CD40L antibody (ab2391)Image courtesy of an anonymous Abreview.ab2391 staining TRAP/CD40L in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-TRAP/CD40L antibody (ab2391)Image courtesy of an AbReview submitted by Dr. Mahesh ShivananjappaImmunofluorescent analysis of paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells TRAP/CD40L with ab2391 at 1/200 dilution, followed by Chicken Anti-Rabbit IgG H&L (Alexa Fluor® 488 secondary antibody at 1/750 dilution (green). WGA is detected with Alexa Fluor® 568 Wheat Germ Agglutinin (red). The nuclear counter stain is DAPI (blue).
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Western blot - Anti-TRAP/CD40L antibody (ab2391)All lanes : Anti-TRAP/CD40L antibody (ab2391)
Lane 1 : Jurkat cell lysates
Lane 2 : Mouse small intestine lysate
Lane 3 : Rat kidney Lysate -
Immunohistochemistry (Frozen sections) - Anti-TRAP/CD40L antibody (ab2391)This image is courtesy of an Abreview submitted by Jens Schmidtab2391 at a 1/100 dilution staining TRAP/CD40L from human inflamed muscle tissue sections by immunohistochemistry (frozen sections). The tissue was acetone fixed (this provides a better result than unfixed tissue) and then incubated with the antibody for 24 hours at 4 °C. Alexa-Fluor ® 594 was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody (ab2391)This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemyab2391 at 1/500 staining rat spleen tissue sections by IHC-P. The tissue was fomraldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor® 488 conjugated goat anti-rabbit was used as the secondary.
Protocols
References
This product has been referenced in:
- Carriba P & Davies AM CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons. Elife 6:N/A (2017). Read more (PubMed: 29111976) »
- Ye QX et al. Indoleamine 2,3-dioxygenase and inducible nitric oxide synthase mediate immune tolerance induced by CTLA4Ig and anti-CD154 hematopoietic stem cell transplantation in a sensitized mouse model. Exp Ther Med 14:1884-1891 (2017). Read more (PubMed: 28962099) »
