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    trapcd40l-antibody-ab2391.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-TRAP/CD40L antibody (ab2391)

  • Datasheet
  • SDS
Reviews (8)Q&A (7)References (7)

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Western blot - Anti-TRAP/CD40L antibody (ab2391)

    Key features and details

    • Rabbit polyclonal to TRAP/CD40L
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    Compound 6877002, CD40-TRAF6 interaction inhibitor (ab146829)
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    Recombinant Dog TRAP/CD40L protein (ab208818)

    View more associated products

    Overview

    • Product name

      Anti-TRAP/CD40L antibody
      See all TRAP/CD40L primary antibodies
    • Description

      Rabbit polyclonal to TRAP/CD40L
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to TRAP/CD40L. surrounding amino acid 246 of human CD40 Ligand

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.03% Proclin 300
      Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Immunoglobulins
      • Class Switching
      • Immunology
      • Innate Immunity
      • Cytokines
      • TNF Superfamily
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Platelets
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Innate and adaptive immunity
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Dog TRAP/CD40L protein (ab208818)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2391 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (2)
    Use a concentration of 1 µg/ml.
    Notes
    WB
    Use a concentration of 1 µg/ml.

    Target

    • Function

      Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
    • Tissue specificity

      Specifically expressed on activated CD4+ T-lymphocytes.
    • Involvement in disease

      Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Post-translational
      modifications

      The soluble form derives from the membrane form by proteolytic processing.
      N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
      Not O-glycosylated.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P29965 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 959 Human
      • Entrez Gene: 21947 Mouse
      • Entrez Gene: 84349 Rat
      • Omim: 300386 Human
      • SwissProt: P29965 Human
      • SwissProt: P27548 Mouse
      • SwissProt: Q9Z2V2 Rat
      • Unigene: 592244 Human
      • Unigene: 4861 Mouse
      • Unigene: 44218 Rat
      see all
    • Alternative names

      • CD 40L antibody
      • CD154 antibody
      • CD40 antigen ligand antibody
      • CD40 ligand antibody
      • CD40 ligand, soluble form antibody
      • CD40-L antibody
      • CD40L antibody
      • CD40L_HUMAN antibody
      • CD40LG antibody
      • gp39 antibody
      • hCD40L antibody
      • HIGM1 antibody
      • IGM antibody
      • IMD3 antibody
      • T B cell activating molecule antibody
      • T BAM antibody
      • T-cell antigen Gp39 antibody
      • TNF-related activation protein antibody
      • TNFSF5 antibody
      • TrAP antibody
      • Tumor necrosis factor (ligand) superfamily member 5 antibody
      • Tumor necrosis factor ligand superfamily member 5 antibody
      see all

    Images

    • Western blot - Anti-TRAP/CD40L antibody (ab2391)
      Western blot - Anti-TRAP/CD40L antibody (ab2391)
      All lanes : Anti-TRAP/CD40L antibody (ab2391)

      Lane 1 : Jurkat cell lysates
      Lane 2 : Mouse small intestine lysate
      Lane 3 : Rat kidney Lysate

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (7)

    Publishing research using ab2391? Please let us know so that we can cite the reference in this datasheet.

    ab2391 has been referenced in 7 publications.

    • Carriba P & Davies AM How CD40L reverse signaling regulates axon and dendrite growth. Cell Mol Life Sci N/A:N/A (2020). PubMed: 32506167
    • Calhan OY  et al. CD40L reverse signaling suppresses prevertebral sympathetic axon growth and tissue innervation. Dev Neurobiol 79:949-962 (2019). PubMed: 32077240
    • Shen DS  et al. Aspirin eugenol ester inhibits agonist-induced platelet aggregation in vitro by regulating PI3K/Akt, MAPK and Sirt 1/CD40L pathways. Eur J Pharmacol 852:1-13 (2019). PubMed: 30797789
    • Howard L  et al. CD40 forward signalling is a physiological regulator of early sensory axon growth. Development 146:N/A (2019). PubMed: 31488565
    • Bagi Z  et al. Extracellular vesicle integrins act as a nexus for platelet adhesion in cerebral microvessels. Sci Rep 9:15847 (2019). PubMed: 31676801
    • Carriba P & Davies AM CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons. Elife 6:N/A (2017). PubMed: 29111976
    • Ye QX  et al. Indoleamine 2,3-dioxygenase and inducible nitric oxide synthase mediate immune tolerance induced by CTLA4Ig and anti-CD154 hematopoietic stem cell transplantation in a sensitized mouse model. Exp Ther Med 14:1884-1891 (2017). PubMed: 28962099

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 15 Abreviews or Q&A

    Question

    Buenos días =,
    Te envio cuestionario sobre anticuerpo ab2391. También te adjunto imágenes de hígado congelado, donde se ve mucha tinción inespecífica, y de tonsil, empleado como control positivo y no se observa tinción.
    Un saludo,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 01 2012

    Answer

    Te agradezco mucho que te hayas tomado la molestia de enviarnos las imágenes y el protocolo.
    Efectivamente, se observa una tinción inespecífica en los tejidos utilizados, y probablemente haya habido algún problema con la alícuota de anticuerpo recibido.
    Espero que la nueva alícuota os funcione como se espera, y en caso contrario no dudéis en contactarnos de nuevo.
    Buen fin de semana.

    Read More

    Abcam Scientific Support

    Answered on Jun 01 2012

    Question

    Telefono: Tincion inespecifica en muestras congeladas de tejido de higado.

    Read More

    Abcam community

    Verified customer

    Asked on May 29 2012

    Answer

    Gracias por habernos contactado y alertado del problema experimentado con nuestro producto.
    Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos.
    Tal y como hemos quedado por conversación telefónica te agradecería mucho que nos enviaras relleno el cuestionario adjunto, en el que se detallan los pasos seguidos durante el protocolo, así como imágenes que muestren la tinción inespecífica del anticuerpo.
    Habréis recibido una confirmación por correo electrónico del nuevo pedido totalmente gratuito del anticuerpo ab65854. El número de pedido es XXXXX. Si necesitáis contactarnos en referencia al mismo no olvidéis mencionar dicho número.
    Espero que este nuevo anticuerpo funcione según lo esperado, en caso contrario, por favor, no dudes en volvernos a contactar.

    Read More

    Abcam Scientific Support

    Answered on May 29 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRAP/CD40L antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (RAW 264.7)
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Specification
    RAW 264.7
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    Submitted May 23 2012

    Immunocytochemistry abreview for Anti-TRAP/CD40L antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Monocytes from Apheresis)
    Permeabilization
    Yes - BD Cytofix/Cytoperm 20 min
    Specification
    Monocytes from Apheresis
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 2°C
    Fixative
    BD Cytofix/Cytoperm 20 min
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    Submitted Feb 02 2012

    Question

    Could you please detail the exact predicted binding sites of the following antibodies: CD40 antibody: ab58612 (Rabbit polyclonal to CD40) - Specifically, where in the C-terminus does this antibody bind - which domain etc? - Can you please issue the sequence of this immunizing peptide? Is it available for purchase? CD40L antibody: ab2391 (Hamster monoclonal to CD40L) - Specifically, where in CD40L does this antibody bind? - Can you please issue the sequence of this immunizing peptide? - Additionally, would you predict that this antibody will recognize mouse CD40L based on the immunizing sequence? CD40L antibody: ab65854 (Rabbit polyclonal to CD40L) - It states that it is raised against a synthetic peptide derived from the N-terminus of human CD40L, but where in the N-terminus does this antibody bind? Can you please issue the sequence of this immunizing peptide? CD40L antibody [MR-1]: ab99894 (Hamster monoclonal to CD40L) - Specifically, which region of CD40L does this antibody interact with? Additionally, can you recommend negative tissue type controls to use for CD40 and CD40L? I would greatly appreciate assistance

    Read More

    Abcam community

    Verified customer

    Asked on Dec 06 2011

    Answer

    Thank you for contacting Abcam regarding our CD40 and CD40L antibodies. Below please find the requested information: ab2391 Rabbit Polyclonal to CD40L - Unfortunately the specific immunogen sequence is proprietary, however I can inform you that it is a small peptide that surrounds aa246 of human CD40L. Also, I have learned from the laboratory that this antibody will react with mouse and is guaranteed to do so. ab58612 Rabbit Polyclonal to CD40 - The specific sequence is proprietary. Unfortunately, the blocking peptide is not commercially available. ab65854 Rabbit Polyclonal to CD40L - Please contact us for additional immunogen information. ab99894 Hamster monoclonal to CD40L - Unfortunately the epitope has not been mapped for this antibody. Regarding a possible negative control - for CD40 and CD40L I would recommend liver. I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Dec 06 2011

    Western blot abreview for Anti-TRAP/CD40L antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Platelets)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    20 µg
    Treatment
    A23187 for 1 h
    Specification
    Platelets
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    Submitted Nov 14 2011

    Flow Cytometry abreview for Anti-TRAP/CD40L antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Platelets)
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Gating Strategy
    Platelets
    Specification
    Platelets
    Preparation
    Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 31 2010

    Western blot abreview for Anti-TRAP/CD40L antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (spinal cord homogenate)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    20 µg
    Treatment
    sham or nerve transected
    Specification
    spinal cord homogenate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 06 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TRAP/CD40L antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spleen)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Ms. Nancy Nutile-McMenemy

    Verified customer

    Submitted Jul 11 2007

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TRAP/CD40L antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (spleen)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Ms. Nancy Nutile-McMenemy

    Verified customer

    Submitted Jul 06 2007

    1-10 of 15 Abreviews or Q&A

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