Key features and details
- Armenian hamster monoclonal [AP-MAB0820] to TRAP/CD40L
- Suitable for: IHC-Fr, Flow Cyt, Inhibition Assay
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-TRAP/CD40L antibody [AP-MAB0820]
See all TRAP/CD40L primary antibodies
DescriptionArmenian hamster monoclonal [AP-MAB0820] to TRAP/CD40L
Host speciesArmenian hamster
Tested applicationsSuitable for: IHC-Fr, Flow Cyt, Inhibition Assaymore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse TRAP/CD40L. Activated Mouse Th1 cells.
General notesThis monoclonal is azide-free & contains an extremely low level of LPS (< 0.002EU/µg IgG).
This hamster monoclonal may be used to inhibit the activation of T and B lymphocytes in vitro and to study antigen-specific lymphocyte responses in vivo.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
Purification notesThe IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography.
Primary antibody notesThis hamster monoclonal may be used to inhibit the activation of T and B lymphocytes in vitro and to study antigen-specific lymphocyte responses in vivo.
Our Abpromise guarantee covers the use of ab83050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
|Inhibition Assay||Use at an assay dependent concentration.|
FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
ab83050 has not yet been referenced specifically in any publications.