Overview

  • Product name
    Anti-TRAP/CD40L antibody [EP462E]
    See all TRAP/CD40L primary antibodies
  • Description
    Rabbit monoclonal [EP462E] to TRAP/CD40L
  • Host species
    Rabbit
  • Specificity
    This antibody reacts with TRAP/CD40L. Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TRAP/CD40L aa 100-200. The exact sequence is proprietary.

  • Positive control
    • WB: MOLT-4 cell lysate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 27882144

Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.

WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
    • Tissue specificity
      Specifically expressed on activated CD4+ T-lymphocytes.
    • Involvement in disease
      Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
    • Sequence similarities
      Belongs to the tumor necrosis factor family.
    • Post-translational
      modifications
      The soluble form derives from the membrane form by proteolytic processing.
      N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
      Not O-glycosylated.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 40L antibody
      • CD154 antibody
      • CD40 antigen ligand antibody
      • CD40 ligand antibody
      • CD40 ligand, soluble form antibody
      • CD40-L antibody
      • CD40L antibody
      • CD40L_HUMAN antibody
      • CD40LG antibody
      • gp39 antibody
      • hCD40L antibody
      • HIGM1 antibody
      • IGM antibody
      • IMD3 antibody
      • T B cell activating molecule antibody
      • T BAM antibody
      • T-cell antigen Gp39 antibody
      • TNF-related activation protein antibody
      • TNFSF5 antibody
      • TrAP antibody
      • Tumor necrosis factor (ligand) superfamily member 5 antibody
      • Tumor necrosis factor ligand superfamily member 5 antibody
      see all

    Images

    • All lanes : Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1 µg/ml

      Lane 1 : Human lymph node tissue lysate
      Lane 2 : Mouse lymph node tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa



      Blocking and diluting buffer: 5% NFDM/TBST. 

      Exposure time: Lane 1: 90 seconds
                               Lane 2: 20 seconds
              

    • Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1/5000 dilution + MOLT-4 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa

    References

    This product has been referenced in:
    • Sun KT  et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep 7:202 (2017). Flow Cyt ; Human . Read more (PubMed: 28303002) »
    • Wang X  et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med 12:3242-3248 (2016). IHC-P ; Rabbit . Read more (PubMed: 27882144) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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