Recombinant
RabMAb

Recombinant Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free (ab210743)

Overview

  • Product name

    Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free
    See all TRAP/CD40L primary antibodies
  • Description

    Rabbit monoclonal [EP462E] to TRAP/CD40L - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TRAP/CD40L aa 100-200. The exact sequence is proprietary.

  • Positive control

    • MOLT-4 cell lysate.
  • General notes

    ab210743 is the carrier-free version of ab52750 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab210743 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab210743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P Use at an assay dependent concentration.

Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.

 

  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
    • Tissue specificity

      Specifically expressed on activated CD4+ T-lymphocytes.
    • Involvement in disease

      Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Post-translational
      modifications

      The soluble form derives from the membrane form by proteolytic processing.
      N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
      Not O-glycosylated.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 40L antibody
      • CD154 antibody
      • CD40 antigen ligand antibody
      • CD40 ligand antibody
      • CD40 ligand, soluble form antibody
      • CD40-L antibody
      • CD40L antibody
      • CD40L_HUMAN antibody
      • CD40LG antibody
      • gp39 antibody
      • hCD40L antibody
      • HIGM1 antibody
      • IGM antibody
      • IMD3 antibody
      • T B cell activating molecule antibody
      • T BAM antibody
      • T-cell antigen Gp39 antibody
      • TNF-related activation protein antibody
      • TNFSF5 antibody
      • TrAP antibody
      • Tumor necrosis factor (ligand) superfamily member 5 antibody
      • Tumor necrosis factor ligand superfamily member 5 antibody
      see all

    Images

    • Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1/5000 dilution + MOLT-4 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 26 kDa
      why is the actual band size different from the predicted?



      This WB data was generated using the same anti-TRAP/CD40L antibody clone, EP462E, in a different buffer formulation (cat# ab52750).

       

    References

    ab210743 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab210743.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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