Overview

  • Product name

    Anti-TRAP/CD40L antibody [MR-1]
    See all TRAP/CD40L primary antibodies
  • Description

    Armenian hamster monoclonal [MR-1] to TRAP/CD40L
  • Host species

    Armenian hamster
  • Specificity

    This antibody detects mouse TRAP/CD40L/CD154. a 39 kDa cell surface glycoprotein expressed predominantly on activated CD4+ lymphocytes.
  • Tested applications

    Suitable for: IHC-P, IP, Functional Studies, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse TRAP/CD40L. activated mouse Th1 clone D1.6

  • Positive control

    • 6-8 hour activated mouse splenocytes
  • General notes


    Functional Application: Blocking T cell costimulation in vivo.

Properties

Applications

Our Abpromise guarantee covers the use of ab99894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 4 µg/ml.

Target

  • Function

    Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • Tissue specificity

    Specifically expressed on activated CD4+ T-lymphocytes.
  • Involvement in disease

    Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 40L antibody
    • CD154 antibody
    • CD40 antigen ligand antibody
    • CD40 ligand antibody
    • CD40 ligand, soluble form antibody
    • CD40-L antibody
    • CD40L antibody
    • CD40L_HUMAN antibody
    • CD40LG antibody
    • gp39 antibody
    • hCD40L antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • T B cell activating molecule antibody
    • T BAM antibody
    • T-cell antigen Gp39 antibody
    • TNF-related activation protein antibody
    • TNFSF5 antibody
    • TrAP antibody
    • Tumor necrosis factor (ligand) superfamily member 5 antibody
    • Tumor necrosis factor ligand superfamily member 5 antibody
    see all

References

ab99894 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting Abcam regarding our CD40 and CD40L antibodies. Below please find the requested information: ab2391 Rabbit Polyclonal to CD40L - Unfortunately the specific immunogen sequence is proprietary, however I can inform you that it is a small peptide that surrounds aa246 of human CD40L. Also, I have learned from the laboratory that this antibody will react with mouse and is guaranteed to do so. ab58612 Rabbit Polyclonal to CD40 - The specific sequence is proprietary. Unfortunately, the blocking peptide is not commercially available. ab65854 Rabbit Polyclonal to CD40L - Please contact us for additional immunogen information. ab99894 Hamster monoclonal to CD40L - Unfortunately the epitope has not been mapped for this antibody. Regarding a possible negative control - for CD40 and CD40L I would recommend liver. I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up