Anti-TRAP/CD40L antibody [MR-1] - Low endotoxin, Azide free (ab99895)
Key features and details
- Armenian hamster monoclonal [MR-1] to TRAP/CD40L - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-TRAP/CD40L antibody [MR-1] - Low endotoxin, Azide free
See all TRAP/CD40L primary antibodies -
Description
Armenian hamster monoclonal [MR-1] to TRAP/CD40L - Low endotoxin, Azide free -
Host species
Armenian hamster -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse TRAP/CD40L. Activated Mouse Th1 clone D1.6
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Positive control
- 6-8 hour activated mouse splenocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MR-1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 4 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 4 µg/ml. |
Target
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Function
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
Tissue specificity
Specifically expressed on activated CD4+ T-lymphocytes. -
Involvement in disease
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21947 Mouse
- SwissProt: P27548 Mouse
- Unigene: 4861 Mouse
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Alternative names
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
Images
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Separation of CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine PMA, ionomycin and LPS stimulated splenocytes stained using ab99895 at 3 μg/ml
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Flow cytometry surface staining pattern of murine PMA, ionomycin and LPS stimulated splenocytes stained using ab99895 at 3 μg/ml
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab99895 has been referenced in 5 publications.
- Hwang Y et al. Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L. Infect Immun 68:511-7 (2000). PubMed: 10639411
- Lode HN et al. Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction. J Clin Invest 105:1623-30 (2000). PubMed: 10841521
- Howard LM et al. Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis. J Clin Invest 103:281-90 (1999). PubMed: 9916140
- Salgado CG et al. Functional CD40 ligand is expressed on epidermal Langerhans cells. J Leukoc Biol 66:281-5 (1999). PubMed: 10449168
- Noelle RJ et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A 89:6550-4 (1992). PubMed: 1378631