Overview

  • Product name

    Trastuzumab ELISA Kit (Herceptin®)
    See all Trastuzumab kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 15%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 15%
  • Sample type

    Serum, Plasma
  • Assay type

    Quantitative
  • Sensitivity

    11 ng/ml
  • Range

    11 ng/ml - 300 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Trastuzumab ELISA Kit (Herceptin®) (ab237645) is a highly specific and sensitive kit designed for the in vitro determination of Trastuzumab in biological matrices such as human serum and plasma. The density of color is proportional to the amount of Rituximab captured from the samples and can be quantified when compared with standard curve.


    Trastuzumab (Herclon®, Herceptin®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). Trastuzumab has anti-tumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. This antibody was approved in 1998 for clinical use for HER2 overexpressing metastatic breast cancer. In HER2 overexpressing cells, trastuzumab markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Assay Buffer 1 x 25ml
    HRP-conjugate Probe 1 x 12ml
    Micro ELISA Plate 1 unit
    Plate sealers 2 units
    Stop Solution 1 x 12ml
    TMB substrate 1 x 12ml
    Trastuzumab Standard S1 1 x 300µl
    Trastuzumab Standard S2 1 x 300µl
    Trastuzumab Standard S3 1 x 300µl
    Trastuzumab Standard S4 1 x 300µl
    Trastuzumab Standard S5 1 x 300µl
    Trastuzumab Standard S6 1 x 300µl
    Trastuzumab Standard S7 1 x 300µl
    Wash buffer (20X) 1 x 50ml
  • Relevance

    Trastuzumab (Herclon®, Herceptin®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). Trastuzumab has antitumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. This antibody was approved in 1998 for clinical use for HER2 overexpressing metastatic breast cancer. In HER2 overexpressing cells, trastuzumab markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes. B
  • Alternative names

    • Herceptin
    • Herclon
    • human epidermal growth factor receptor 2

Applications

Our Abpromise guarantee covers the use of ab237645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

Protocols

References

ab237645 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237645.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up