Overview

  • Product name

    anti-Trastuzumab ELISA Kit (Herceptin®)
    See all Trastuzumab kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 15%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 15%
  • Sample type

    Serum, Plasma
  • Assay type

    Quantitative
  • Sensitivity

    30 ng/ml
  • Range

    62 ng/ml - 500 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    anti-Trastuzumab ELISA Kit (Herceptin®) (ab237655) is designed to quantify/measure the antibody against Trastuzumab with high specificity and sensitivity in biological matrices.


    Trastuzumab (Herclon®, Herceptin®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). Trastuzumab has anti-tumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. In HER2 overexpressing cells, Trastuzumab markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug-antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Assay Buffer 1 x 50ml
    Confirmation Reagent 1 x 12ml
    Micro ELISA Plate 1 unit
    Peroxidase Conjugate 1 x 12ml
    Plate sealers 2 units
    Stop Solution 1 x 12ml
    TMB substrate 1 x 12ml
    Trastuzumab Standard S1 1 x 1ml
    Trastuzumab Standard S2 1 x 1ml
    Trastuzumab Standard S3 1 x 1ml
    Trastuzumab Standard S4 1 x 1ml
    Trastuzumab Standard S5 1 x 1ml
    Trastuzumab Standard S6 1 x 1ml
    Trastuzumab Standard S7 1 x 1ml
    Wash buffer (20X) 1 x 50ml
  • Relevance

    Trastuzumab (Herclon®, Herceptin®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). Trastuzumab has antitumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. This antibody was approved in 1998 for clinical use for HER2 overexpressing metastatic breast cancer. In HER2 overexpressing cells, trastuzumab markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes. B
  • Alternative names

    • Herceptin
    • Herclon
    • human epidermal growth factor receptor 2

Applications

Our Abpromise guarantee covers the use of ab237655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

Protocols

References

ab237655 has not yet been referenced specifically in any publications.

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