Recombinant Anti-TRAX antibody [EPR13929(B)] (ab181862)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13929(B)] to TRAX
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TRAX antibody [EPR13929(B)]
See all TRAX primary antibodies -
Description
Rabbit monoclonal [EPR13929(B)] to TRAX -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat testis lysates. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13929(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/20000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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ICC/IF |
1/100.
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IP |
1/60.
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Notes |
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WB
1/1000 - 1/20000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
ICC/IF
1/100. |
IP
1/60. |
Target
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Function
Possible role in spermatogenesis. -
Sequence similarities
Belongs to the translin family. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cytoplasm > perinuclear region. Nucleus. Expressed in the cytoplasm in the presence of TSN. - Information by UniProt
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Database links
- Entrez Gene: 7257 Human
- Entrez Gene: 100039215 Mouse
- Entrez Gene: 53424 Mouse
- Entrez Gene: 64028 Rat
- Omim: 602964 Human
- SwissProt: Q99598 Human
- SwissProt: Q9QZE7 Mouse
- SwissProt: Q9JHB5 Rat
see all -
Alternative names
- Translin associated factor X antibody
- Translin like protein antibody
- Translin-associated factor X antibody
see all
Images
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Anti-TRAX antibody [EPR13929(B)] (ab181862) at 1/5000 dilution + Human testis lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 33 kDa -
Western blot analysis of immunoprecipitation pellet from Human testis lysate immunoprecipitated using ab181862 at 1/60 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution. -
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling TRAX with ab181862 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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All lanes : Anti-TRAX antibody [EPR13929(B)] (ab181862) at 1/20000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 33 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181862 has not yet been referenced specifically in any publications.