Overview

  • Product name
    Anti-TRBP antibody [EPR13550]
    See all TRBP primary antibodies
  • Description
    Rabbit monoclonal [EPR13550] to TRBP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TRBP aa 200-300. The exact sequence is proprietary.
    Database link: Q15633

  • Positive control
    • Jurkat, HeLa, HepG2 and MCF-7 cell lysates; Human Infiltrating duct carcinoma of breast tissue; HeLa cells; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50.
IP 1/40.
IHC-P 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • Function
    Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.
  • Sequence similarities
    Contains 3 DRBM (double-stranded RNA-binding) domains.
  • Cellular localization
    Cytoplasm. Cytoplasm > perinuclear region. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOQS antibody
    • Prbp antibody
    • RISC loading complex subunit TARBP2 antibody
    • RISC-loading complex subunit tarbp2 antibody
    • TAR (HIV 1) RNA binding protein 2 antibody
    • TAR (HIV) RNA binding protein 2 antibody
    • TAR (HIV) RNA-binding protein TRBP1 antibody
    • TAR RNA Binding Protein 2 antibody
    • TAR RNA-binding protein 2 antibody
    • tarbp2 antibody
    • TARBP2 RISC loading complex RNA binding subunit antibody
    • Trans-activation-responsive RNA-binding protein antibody
    • TRBP antibody
    • TRBP1 antibody
    • TRBP2 antibody
    • TRBP2_HUMAN antibody
    see all

Images

  • All lanes : Anti-TRBP antibody [EPR13550] (ab180947) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

  • ab180947 staining TRBP antibody in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

  • Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180947 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TRBP with ab180947 at 1/50 dilution (red) compared to a Rabbit monoclonal IgG Isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunofluorescent analysis of acetone-fixed HeLa cells labeling TRBP with ab180947 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). DAPI staining (blue).

  • Immunohistochemical analysis of paraffin-embedded Human Infiltrating duct carcinoma of breast tissue labeling TRBP with ab180947 at 1/250 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

ab180947 has not yet been referenced specifically in any publications.

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