Product nameAnti-TRBP antibody [EPR13550]
See all TRBP primary antibodies
DescriptionRabbit monoclonal [EPR13550] to TRBP
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TRBP aa 200-300. The exact sequence is proprietary.
Database link: Q15633
- Jurkat, HeLa, HepG2 and MCF-7 cell lysates; Human Infiltrating duct carcinoma of breast tissue; HeLa cells; Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab180947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
FunctionRequired for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.
Sequence similaritiesContains 3 DRBM (double-stranded RNA-binding) domains.
Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Nucleus.
- Information by UniProt
- LOQS antibody
- Prbp antibody
- RISC loading complex subunit TARBP2 antibody
All lanes : Anti-TRBP antibody [EPR13550] (ab180947) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
ab180947 staining TRBP antibody in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control 1:PBS only.
Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180947 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TRBP with ab180947 at 1/50 dilution (red) compared to a Rabbit monoclonal IgG Isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunofluorescent analysis of acetone-fixed HeLa cells labeling TRBP with ab180947 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). DAPI staining (blue).
Immunohistochemical analysis of paraffin-embedded Human Infiltrating duct carcinoma of breast tissue labeling TRBP with ab180947 at 1/250 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab180947 has been referenced in 1 publication.
- Tian T et al. Long Noncoding RNA MPRL Promotes Mitochondrial Fission and Cisplatin Chemosensitivity via Disruption of Pre-miRNA Processing. Clin Cancer Res 25:3673-3688 (2019). PubMed: 30885939