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RecombinantRabMAb

Recombinant Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)

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Western blot - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
  • Immunoprecipitation - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
  • Flow Cytometry (Intracellular) - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
  • Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13550] to TRBP - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

PE Unconjugated

Overview

  • Product name

    Anti-TRBP antibody [EPR13550] - BSA and Azide free
    See all TRBP primary antibodies

  • Description

    Rabbit monoclonal [EPR13550] to TRBP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, Flow Cyt (Intra), WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250279 is the carrier-free version of ab180947.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.

  • Sequence similarities

    Contains 3 DRBM (double-stranded RNA-binding) domains.

  • Cellular localization

    Cytoplasm. Cytoplasm > perinuclear region. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • LOQS antibody
    • Prbp antibody
    • RISC loading complex subunit TARBP2 antibody
    • RISC-loading complex subunit tarbp2 antibody
    • TAR (HIV 1) RNA binding protein 2 antibody
    • TAR (HIV) RNA binding protein 2 antibody
    • TAR (HIV) RNA-binding protein TRBP1 antibody
    • TAR RNA Binding Protein 2 antibody
    • TAR RNA-binding protein 2 antibody
    • tarbp2 antibody
    • TARBP2 RISC loading complex RNA binding subunit antibody
    • Trans-activation-responsive RNA-binding protein antibody
    • TRBP antibody
    • TRBP1 antibody
    • TRBP2 antibody
    • TRBP2_HUMAN antibody
    see all

Images

  • Western blot - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    Western blot - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    All lanes : Anti-TRBP antibody [EPR13550] (ab180947) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    This data was developed using ab180947, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    This data was developed using ab180947, the same antibody clone in a different buffer formulation.ab180947 staining TRBP antibody in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain. Negative control 1:PBS only.
  • Immunoprecipitation - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    Immunoprecipitation - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    This data was developed using ab180947, the same antibody clone in a different buffer formulation.Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180947 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution.
  • Flow Cytometry (Intracellular) - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    Flow Cytometry (Intracellular) - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)

    This data was developed using ab180947, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TRBP with ab180947 at 1/50 dilution (red) compared to a Rabbit monoclonal IgG Isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    This data was developed using ab180947, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed HeLa cells labeling TRBP with ab180947 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). DAPI staining (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    This data was developed using ab180947, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Infiltrating duct carcinoma of breast tissue labeling TRBP with ab180947 at 1/250 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)
    Anti-TRBP antibody [EPR13550] - BSA and Azide free (ab250279)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab250279? Please let us know so that we can cite the reference in this datasheet.

ab250279 has not yet been referenced specifically in any publications.

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