Overview

  • Product name
    Anti-TRC40 antibody [EPR11422]
    See all TRC40 primary antibodies
  • Description
    Rabbit monoclonal [EPR11422] to TRC40
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRC40 aa 50-150.

  • Positive control
    • Fetal brain, fetal kidney, HeLa and 293T lysates; Human cervical carcinoma and kidney tissues; Immunoprecipitation pellet from fetal brain lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). May be involved in insulin signaling.
    • Tissue specificity
      Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle.
    • Sequence similarities
      Belongs to the arsA ATPase family.
    • Cellular localization
      Cytoplasm. Endoplasmic reticulum. Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARSA 1 antibody
      • ARSA antibody
      • ArsA arsenite transporter ATP binding homolog 1 antibody
      • ArsA arsenite transporter, ATP binding, E. coli, homolog of, 1 antibody
      • ArsA arsenite transporter, ATP-binding, homolog 1 (bacterial) antibody
      • ARSA I antibody
      • ARSA-I antibody
      • ARSA1 antibody
      • ARSAI antibody
      • Arsenical pump driving ATPase antibody
      • Arsenical pump-driving ATPase antibody
      • Arsenical resistance ATPase antibody
      • Arsenite translocating ATPase antibody
      • Arsenite transporting ATPase antibody
      • Arsenite-stimulated ATPase antibody
      • ASNA 1 antibody
      • ASNA I antibody
      • ASNA-I antibody
      • ASNA_HUMAN antibody
      • ASNA1 antibody
      • Asna1 protein antibody
      • ASNAI antibody
      • ATPase ASNA1 antibody
      • GET3 antibody
      • Golgi to ER traffic 3 homolog antibody
      • hARSA-I antibody
      • hASNA-I antibody
      • MGC3821 antibody
      • Transmembrane domain recognition complex 40 kDa ATPase subunit antibody
      • Transmembrane domain recognition complex, 40kDa antibody
      • TRC40 antibody
      see all

    Images

    • All lanes : Anti-TRC40 antibody [EPR11422] (ab169549) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Fetal kidney lysate
      Lane 3 : HeLa lysate
      Lane 4 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    • Anti-TRC40 antibody [EPR11422] (ab169549) at 1/1000 dilution + immunoprecipitation pellet from fetal brain lysate

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

      Predicted band size: 39 kDa

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling TRC40 with ab169549 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TRC40 with ab169549 at 1/50 dilution.

    References

    ab169549 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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