Recombinant Anti-TRC40 antibody [EPR11423] (ab169539)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11423] to TRC40
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-TRC40 antibody [EPR11423]
See all TRC40 primary antibodies -
Description
Rabbit monoclonal [EPR11423] to TRC40 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human TRC40 aa 300-400. The exact sequence is proprietary.
Database link: O43681 -
Positive control
- Fetal brain, fetal kidney, HeLa and 293T lysates. Human colon and Human lung tissue. HeLa and permeabilized 293T cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11423 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/250 - 1/500.
|
|
IP |
1/10 - 1/100.
|
Notes |
---|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
IP
1/10 - 1/100. |
Target
-
Function
ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). May be involved in insulin signaling. -
Tissue specificity
Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle. -
Sequence similarities
Belongs to the arsA ATPase family. -
Cellular localization
Cytoplasm. Endoplasmic reticulum. Nucleus > nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 439 Human
- Entrez Gene: 56495 Mouse
- Entrez Gene: 288919 Rat
- GenBank: NP_004308.2 Human
- Omim: 601913 Human
- SwissProt: O43681 Human
- SwissProt: O54984 Mouse
- Unigene: 465985 Human
see all -
Alternative names
- ARSA 1 antibody
- ARSA antibody
- ArsA arsenite transporter ATP binding homolog 1 antibody
see all
Images
-
All lanes : Anti-TRC40 antibody [EPR11423] (ab169539) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Fetal kidney lysate
Lane 3 : HeLa lysate
Lane 4 : 293T lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa -
Immunoprecipitation. ab169539 at 1/10000 staining TRC40 in 293T cell lysate immunoprecipitated using ab169539 at 1/10. Predicted band size : 39 kDa.
-
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TRC40 with ab169539 antibody at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human lung tissue labeling TRC40 with ab169539 antibody at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunofluorescent analysis of HeLa cells labeling TRC40 with ab169539 at 1/250.
-
Intracellular flow cytometrical analysis of permeabilized 293T cells with ab169539 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab169539 has not yet been referenced specifically in any publications.