Anti-TRC8 antibody - C-terminal (ab223683)
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Overview
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Product name
Anti-TRC8 antibody - C-terminal
See all TRC8 primary antibodies -
Description
Goat polyclonal to TRC8 - C-terminal -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide corresponding to Human TRC8 aa 651-663 (C terminal) (Cysteine residue). NP_009149.2.
Sequence:C-HTGAAAEEFNDDT
Database link: Q8WU17 -
Positive control
- WB: Human skeletal muscle lysate. IHC-P: Human placenta tissue.
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General notes
This product was previously labelled as RNF139
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223683 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab223683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa). | |
| IHC-P | Use a concentration of 3 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
RNF139 is a multi-membrane spanning protein E3 ubiquitin ligase which is mutated in clear-cell renal carcinomas. It contains a sterol-sensing domain and a RING-H2 motif essential for ubiquitin ligase activity. It is a potential tumor suppressor for renal cell carcinoma. Human kidney cells are growth inhibited by RNF139. Inhibition is manifested by G2/M arrest and is dependent upon the ubiquitin ligase activity of the RING domain. Expression of RNF139 represses genes involved in cholesterol and fatty acid biosynthesis that are transcriptionally regulated by the sterol response element binding proteins (SREBPs). RNF139 modulation of SREBP activity comprises a novel regulatory link between growth control and the cholesterol/lipid homeostasis pathway. -
Cellular localization
Endoplasmic reticulum membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 11236 Human
- Omim: 603046 Human
- SwissProt: Q8WU17 Human
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Alternative names
- E3 ubiquitin-protein ligase RNF139 antibody
- HRCA1 antibody
- RCA1 antibody
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Images
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Western blot - Anti-TRC8 antibody - C-terminal (ab223683)Anti-TRC8 antibody - C-terminal (ab223683) at 1 µg/ml + Human skeletal muscle lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC8 antibody - C-terminal (ab223683)Paraffin-embedded human placenta tissue stained for TRC8 using ab223683 at 3.8 µg/ml in immunohistochemical analysis, followed by AP-staining.
Datasheets and documents
References
ab223683 has not yet been referenced specifically in any publications.
