Product nameAnti-Trefoil Factor 3 antibody
See all Trefoil Factor 3 primary antibodies
DescriptionRabbit polyclonal to Trefoil Factor 3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human Trefoil Factor 3 (C terminal). The exact sequence is proprietary.
Database link: Q07654
- IHC-P: Mouse colon and intestine tissue. WB: MCF7 and MDA-MB-231 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Tissue specificityExpressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
Sequence similaritiesContains 1 P-type (trefoil) domain.
Cellular localizationSecreted > extracellular space > extracellular matrix > apical lamina.
- Information by UniProt
- hITF antibody
- hP1.B antibody
- Intestinal trefoil factor antibody
Paraffin-embedded mouse colon tissue stained for Trefoil Factor 3 using ab272927 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
All lanes : Anti-Trefoil Factor 3 antibody (ab272927) at 1/1000 dilution
Lane 1 : MCF7 whole cell extract
Lane 2 : MDA-MB-231 whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Paraffin-embedded mouse intestine tissue stained for Trefoil Factor 3 using ab272927 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272927 has not yet been referenced specifically in any publications.