Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3973] to Trefoil Factor 3 - BSA and Azide free
- Suitable for: ICC, IHC-P, Flow Cyt, WB
- Reacts with: Human
Product nameAnti-Trefoil Factor 3 antibody [EPR3973] - BSA and Azide free
See all Trefoil Factor 3 primary antibodies
DescriptionRabbit monoclonal [EPR3973] to Trefoil Factor 3 - BSA and Azide free
Tested applicationsSuitable for: ICC, IHC-P, Flow Cyt, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human Trefoil Factor 3 aa 1-100. The exact sequence is proprietary.
ab247760 is the carrier-free version of ab109104 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247760 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab247760 in the following tested applications.
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 9 kDa.|
FunctionInvolved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Tissue specificityExpressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
Sequence similaritiesContains 1 P-type (trefoil) domain.
Cellular localizationSecreted > extracellular space > extracellular matrix > apical lamina.
- Information by UniProt
- hITF antibody
- hP1.B antibody
- Intestinal trefoil factor antibody
This data was developed using ab109104, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Trefoil Factor 3 in paraffin-embedded Human colon tissue using ab109104 at 1/500 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104) at 1/1000 dilution
Lane 1 : Human uterus cancer lysate
Lane 2 : Human colon cancer lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 9 kDa
This data was developed using ab109104, the same antibody clone in a different buffer formulation.
This data was developed using ab109104, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of HT-29 (human colorectal adenocarcinoma) cells labeling Trefoil Factor 3 with unpurified ab109104 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247760 has not yet been referenced specifically in any publications.