Overview

  • Product name

  • Description

    Rabbit polyclonal to TREM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length Human TREM1 (NP_061113.1, amino acids 1-234).

  • Positive control

    • Mouse kidney tissue lysate; TREM1 transfected 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.

Target

  • Function

    Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.
  • Tissue specificity

    Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD354 antibody
    • OTTMUSP00000018206 antibody
    • TREM 1 antibody
    • TREM-1 antibody
    • TREM1 antibody
    • TREM1_HUMAN antibody
    • Triggering receptor expressed on monocytes 1 antibody
    • Triggering receptor expressed on myeloid cells 1 antibody
    • Triggering receptor TREM 1 antibody
    • Triggering receptor TREM1 antibody
    see all

Images

  • Anti-TREM1 antibody (ab104413) at 1 µg/ml + Mouse kidney tissue lysate at 50 µg

    Predicted band size: 26 kDa

  • All lanes : Anti-TREM1 antibody (ab104413) at 1 µg/ml

    Lane 1 : TREM1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 26 kDa

References

This product has been referenced in:

  • Liu F  et al. TREM1: A positive regulator for inflammatory response via NF-?B pathway in A549 cells infected with Mycoplasma pneumoniae. Biomed Pharmacother 107:1466-1472 (2018). Read more (PubMed: 30257363) »
  • Shi R  et al. Expression level of 12-amino acid triggering receptor on myeloid cells-like transcript 1 derived peptide alleviates lipopolysaccharide-induced acute lung injury in mice. Int J Mol Med 41:2159-2168 (2018). Read more (PubMed: 29393375) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (peritoneal macrophage)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Treatment
LPS
Specification
peritoneal macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Mei Liang

Verified customer

Submitted Aug 28 2019

Question
Answer

DISCOUNT CODE: ***
Expiration date: ***

I am very pleased to hear you would like to accept our offer and test ab104413 in flow cytometry or immunocytochemistry. To redeem this offer, please submit an Abreview for ICC or FC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
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We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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