Overview

  • Product name

  • Description

    Goat polyclonal to TREM2
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    C-QVEHSTSRNQET

    , corresponding to internal sequence amino acids 154-165 of Mouse TREM2 (NP_112544.1).

  • Positive control

    • Mouse liver lysate IF/ICC: RAW 246.7 cell line.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab95470 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
  • Tissue specificity

    Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
  • Involvement in disease

    Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • TREM 2 antibody
    • TREM-2 antibody
    • TREM2 antibody
    • TREM2_HUMAN antibody
    • TREM2a antibody
    • TREM2b antibody
    • TREM2c antibody
    • Trggering receptor expressed on myeloid cells 2 antibody
    • Trggering receptor expressed on myeloid cells 2a antibody
    • Triggering receptor expressed on monocytes 2 antibody
    • Triggering receptor expressed on myeloid cells 2 antibody
    see all

Images

  • Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation with ab95470 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

  • ab95470 stained RAW 246.7 cells. The cells were 100% methanol fixed for 5 minutes at-20°C and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95470 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150133) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Immunocytochemical immunoflurescence analysis of PFA-fixed human neuroblastoma cell line, labelling TREM2 using ab95470 at a dilution of 1/200 incubated for 2 hours and 24°C in 0.1M PBST with 10% donkey serum. Permeablisation was accomplished using 0.1M PBS with 1% Triton X. Blocking was done with 10% donkey serum in PBST and incubated for 30 minutes at 24°C. The secondary used was a polyclonal donkey anti-goat Alexa Fluor® 568 conjugate.

    See Abreview

References

This product has been referenced in:

  • Guo Y  et al. TREM2 deficiency aggravates a-synuclein-induced neurodegeneration and neuroinflammation in Parkinson's disease models. FASEB J N/A:fj201900992R (2019). Read more (PubMed: 31370707) »
  • Jendresen C  et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). Read more (PubMed: 28320424) »
See all 3 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
Yes - Triton-x100
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Mr. Faheem Ullah

Verified customer

Submitted May 22 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1xPBS with 1% TritonX100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 17 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Neuroblastoma cell line)
Permeabilization
Yes - 0.1 M PBS with 1% Triton X
Specification
Neuroblastoma cell line
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 20 2015

Application
Western blot
Sample
Human Serum (Human serum)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
Human serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 20 2015

Answer

Vielen Dank für Ihre Email.


Wie besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und sollte Freitag bei Ihnen ankommen.

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Unser Garantiezeitraum beträgt6 Monate, und eigentlich kann ich bei einer Bestellung vom Januar 2011kein Auge zudrücken.

Da ichIhnen allerdings schon einen Ersatz zugesagt habe und es versäumt habe,den Garantiezeitraum zu erwähnen, werde ich Ihnen ausnahmsweise einen Antikörper Ihrer Wahl zukommen lassen.

Eine Gutschrift kann ich Ihnen nicht zukommen lassen, da die Bestellung so weit außerhalb besagter Garantie liegt, und da hat die Finanzabteilung das letzte Wort.

Ich hoffe, dies hilft Ihnen trotzdem weiter. Bittelassen Sie mich wissen, welcher Antikörper für Sie als Alternative in Betracht kommt.

Vielen dank für Ihr Verstandniss.

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Answer

Vielen Dank für Ihre Geduld.

Da der Antikörper nicht so funktioniert wie er sollte, werden wir Ihnen gerne einen kostenlosen Ersatz senden, Ihnen eine Gutschrift ausstellen oder den Antikörper erstatten.

Wir müssten allerdings noch wissen, wann Sie den Antikörper bei uns bestellt haben, um Ihre Bestellung im System zu finden.

Des weiteren denke ich nicht, dass wir es hier mit einem Antikörper zu tun haben, der in der KO Maus detektiert: Der Antikörper reagiert in diesem Fall einfach nur komplett unspezifisch, da er aus unbekannten Gründen kaputt gegangen ist. Sie brauchen sich daher auch nicht die Mühe machen, ihn an uns zurück zu senden.

Desweiteren möchte ich Sie noch dringendbitten, Ihr Protokoll hinsichtlich des Blockmittels abzuändern, wenn Sie einen Primär- Antikörper aus der Ziege verwenden:

Der sekundäre Antikörper kreuzreagiert mitden bovinen IgG des BSA (das gleiche gilt zu einem Gewissen Grad auch fuerdie Milch), was zu einem erhöhten Hintergrund führt, da BSA als Maskierungsmittel sichebenfallsandie Membran bindet. Verwenden Sie wen möglich das Serum der Spezies, aus dem der Sekundär- AK gewonnen wurde.

Dies gilt immer für primäre Antikörper, die aus der Ziege, dem Schaf und aus dem Pferd gewonnen wurden.



Bitte lassen Sie mich wissen, wann Sie den Antikörper bei uns bestellt haben.

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