Key features and details
- Goat polyclonal to TREM2
- Suitable for: ICC
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-TREM2 antibody
See all TREM2 primary antibodies
DescriptionGoat polyclonal to TREM2
Tested applicationsSuitable for: ICCmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat
- ICC: NIH3T3-L1 cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab95470 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab95470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 10 mg/ml.|
FunctionMay have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
Tissue specificityExpressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
Involvement in diseaseDefects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- TREM 2 antibody
- TREM-2 antibody
- TREM2 antibody
Immunocytochemistry/ Immunofluorescence analysis of paraformaldehyde fixed mouse 3T3-L1 cells labeling TREM2 with ab95470 at 10 µg/mL (incubation time, 1 hour). Cells were permeabilized with 0.15% Triton. Alexa Fluor 488 secondary antibody was used at 2µg/mL. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 µg/mL followed by Alexa Fluor 488 secondary antibody 2 µg/mL.
Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation with ab95470 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
ab95470 has been referenced in 5 publications.
- Guo Y et al. TREM2 deficiency aggravates a-synuclein-induced neurodegeneration and neuroinflammation in Parkinson's disease models. FASEB J N/A:fj201900992R (2019). PubMed: 31370707
- Ji J et al. The Intra-nuclear SphK2-S1P Axis Facilitates M1-to-M2 Shift of Microglia via Suppressing HDAC1-Mediated KLF4 Deacetylation. Front Immunol 10:1241 (2019). PubMed: 31214192
- Wang ECE et al. A Subset of TREM2+ Dermal Macrophages Secretes Oncostatin M to Maintain Hair Follicle Stem Cell Quiescence and Inhibit Hair Growth. Cell Stem Cell 24:654-669.e6 (2019). PubMed: 30930146
- Jendresen C et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). PubMed: 28320424
- Raha AA et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). WB, IHC ; Mouse . PubMed: 27662313