Product nameAnti-TREM2 antibody [EPR20243]
See all TREM2 primary antibodies
DescriptionRabbit monoclonal [EPR20243] to TREM2
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human TREM2 aa 1-200. The exact sequence is proprietary.
Database link: Q9NZC2
- WB: THP-1 whole cell lysate treated with 100ng/ml Phorbol-12-myristate-13-acetate (PMA) for 72 hours. ICC/IF: SK-N-F1 cell line. IP: THP-1 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa).|
FunctionMay have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
Tissue specificityExpressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
Involvement in diseaseDefects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- TREM 2 antibody
- TREM-2 antibody
- TREM2 antibody
All lanes : Anti-TREM2 antibody [EPR20243] (ab209814) at 1/1000 dilution
Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 whole cell lysate treated with 100ng/ml Phorbol-12-myristate-13-acetate (PMA) for 72 hours
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 4 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-N-F1 cells labeling TREM2 with ab209814 at 1/500 dilution, followed by Donkey anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (green).
The nuclear counter stain is DAPI (blue).
TREM2 was immunoprecipitated from 0.35 mg of THP-1 (Human monocytic leukemia cell line) whole cell lysate with ab209814 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209814 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: THP-1 whole cell lysate, 10 μg (Input).
Lane 2: ab209814 IP in THP-1 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209814 in THP-1 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This product has been referenced in:
- Raha-Chowdhury R et al. Choroid Plexus Acts as Gatekeeper for TREM2, Abnormal Accumulation of ApoE, and Fibrillary Tau in Alzheimer's Disease and in Down Syndrome Dementia. J Alzheimers Dis 69:91-109 (2019). Read more (PubMed: 30909239) »
- Raha-Chowdhury R et al. Erythromyeloid-Derived TREM2: A Major Determinant of Alzheimer's Disease Pathology in Down Syndrome. J Alzheimers Dis 61:1143-1162 (2018). Read more (PubMed: 29278889) »