Overview

  • Product name

    Anti-TREM2 antibody [EPR20243]
    See all TREM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20243] to TREM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TREM2 aa 1-200. The exact sequence is proprietary.
    Database link: Q9NZC2

  • Positive control

    • WB: THP-1 whole cell lysate treated with 100ng/ml Phorbol-12-myristate-13-acetate (PMA) for 72 hours. ICC/IF: SK-N-F1 cell line. IP: THP-1 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20243
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab209814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IP 1/40.
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa).

Target

  • Function

    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
  • Tissue specificity

    Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
  • Involvement in disease

    Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • TREM 2 antibody
    • TREM-2 antibody
    • TREM2 antibody
    • TREM2_HUMAN antibody
    • TREM2a antibody
    • TREM2b antibody
    • TREM2c antibody
    • Trggering receptor expressed on myeloid cells 2 antibody
    • Trggering receptor expressed on myeloid cells 2a antibody
    • Triggering receptor expressed on monocytes 2 antibody
    • Triggering receptor expressed on myeloid cells 2 antibody
    see all

Images

  • All lanes : Anti-TREM2 antibody [EPR20243] (ab209814) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 2 : THP-1 whole cell lysate treated with 100ng/ml Phorbol-12-myristate-13-acetate (PMA) for 72 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 25 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-N-F1 cells labeling TREM2 with ab209814 at 1/500 dilution, followed by Donkey anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (green).

    The nuclear counter stain is DAPI (blue).

  • TREM2 was immunoprecipitated from 0.35 mg of THP-1 (Human monocytic leukemia cell line) whole cell lysate with ab209814 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209814 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: THP-1 whole cell lysate, 10 μg (Input).

    Lane 2: ab209814 IP in THP-1 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209814 in THP-1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

This product has been referenced in:

  • Raha-Chowdhury R  et al. Choroid Plexus Acts as Gatekeeper for TREM2, Abnormal Accumulation of ApoE, and Fibrillary Tau in Alzheimer's Disease and in Down Syndrome Dementia. J Alzheimers Dis 69:91-109 (2019). Read more (PubMed: 30909239) »
  • Raha-Chowdhury R  et al. Erythromyeloid-Derived TREM2: A Major Determinant of Alzheimer's Disease Pathology in Down Syndrome. J Alzheimers Dis 61:1143-1162 (2018). Read more (PubMed: 29278889) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Serum (Human serum)
Gel Running Conditions
Reduced Denaturing (12% SDS)
Loading amount
20 µg
Specification
Human serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jan 19 2018

Application
Immunohistochemistry free floating
Sample
Human Tissue sections (Human Brain)
Specification
Human Brain

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jan 19 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Neuroblastoma)
Permeabilization
Yes - 1% Triton X with 0.1M PBS
Specification
Neuroblastoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Methanol

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jan 19 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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