Recombinant Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)
![Immunoprecipitation - Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)](https://www.abcam.com/ps/products/232/ab232470/Images/ab232470-321124-anti-trem2-antibody-epr20243-immunoprecipitation.jpg "Immunoprecipitation - Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20243] to TREM2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-TREM2 antibody [EPR20243] - BSA and Azide free
See all TREM2 primary antibodies -
Description
Rabbit monoclonal [EPR20243] to TREM2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human TREM2 aa 1-200. The exact sequence is proprietary.
Database link: Q9NZC2 -
General notes
Ab232470 is the carrier-free version of ab209814. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232470 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20243 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab232470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. -
Tissue specificity
Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. -
Involvement in disease
Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 54209 Human
- Omim: 605086 Human
- SwissProt: Q9NZC2 Human
- Unigene: 435295 Human
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Alternative names
- TREM 2 antibody
- TREM-2 antibody
- TREM2 antibody
see all
Images
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Immunoprecipitation - Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)
TREM2 was immunoprecipitated from 0.35 mg of THP-1 (Human monocytic leukemia cell line) whole cell lysate with ab209814 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209814 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: THP-1 whole cell lysate, 10 μg (Input).
Lane 2: ab209814 IP in THP-1 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209814 in THP-1 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209814).
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![Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)](https://www.abcam.com/ps/products/232/ab232470/Images/ab232470-3-benefits-of-recombinant-antibodies.png)
Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab232470 has not yet been referenced specifically in any publications.
