Recombinant Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)
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Overview
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Product name
Anti-TREM2 antibody [EPR20243] - BSA and Azide free
See all TREM2 primary antibodies -
Description
Rabbit monoclonal [EPR20243] to TREM2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human TREM2 aa 1-200. The exact sequence is proprietary.
Database link: Q9NZC2 -
Positive control
- ICC/IF: SK-N-F1 cells.
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General notes
Ab232470 is the carrier-free version of ab209814. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232470 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20243 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab232470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. -
Tissue specificity
Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. -
Involvement in disease
Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 54209 Human
- Omim: 605086 Human
- SwissProt: Q9NZC2 Human
- Unigene: 435295 Human
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Alternative names
- TREM 2 antibody
- TREM-2 antibody
- TREM2 antibody
see all
Images
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![Immunoprecipitation - Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)](https://www.abcam.com/ps/products/232/ab232470/Images/ab232470-321124-anti-trem2-antibody-epr20243-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)TREM2 was immunoprecipitated from 0.35 mg of THP-1 (Human monocytic leukemia cell line) whole cell lysate with ab209814 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209814 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: THP-1 whole cell lysate, 10 μg (Input).
Lane 2: ab209814 IP in THP-1 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209814 in THP-1 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209814).
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![Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)](https://www.abcam.com/ps/products/232/ab232470/Images/ab232470-308872-anti-trem2-antibody-epr20243-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-N-F1 cells labeling TREM2 with ab209814 at 1/500 dilution, followed by Donkey anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (green).
The nuclear counter stain is DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209814).
Datasheets and documents
References
ab232470 has not yet been referenced specifically in any publications.
