Key features and details
- Rat monoclonal [RM0139-5J46] to TREM2
- Suitable for: ICC, IHC-P
- Reacts with: Mouse
- Isotype: IgG2b
Product nameAnti-TREM2 antibody [RM0139-5J46]
See all TREM2 primary antibodies
DescriptionRat monoclonal [RM0139-5J46] to TREM2
Tested applicationsSuitable for: ICC, IHC-Pmore details
Species reactivityReacts with: Mouse
Mouse recombinant protein of the extracellular EC-domain of TREM2
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Purification notesThe IgG fraction of culture supernatant was purified by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab86491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/50 - 1/100.|
|IHC-P||Use at an assay dependent concentration.|
FunctionMay have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
Tissue specificityExpressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
Involvement in diseaseDefects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- TREM 2 antibody
- TREM-2 antibody
- TREM2 antibody
Immunocytochemistry analysis of mouse brain microglia cells labeling TREM2 antibody with ab86491.
IHC-Fr image of human blood vessels stained with Ab86491 (1:250). The fixed brain sections were permeabilized using 0.1% PBS-Triton X for 1h. 10% donkey serum was used for 1 hour at 24°C to block protein-protein interactions. The sections were then incubated with Ab8649 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-rat used at 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence analysis of 18 day rat embroyonic hypothalmic neurones labelling TREM2 with ab86491. Embryonic hypothalmic neuronal cells were cultured in a neurobasal media with B27 culture suppliment for three weeks and fixed with 2%PFA. A rat monoclonal Alexa fluoro® 568 (red) secondary was used at a concentration of 1/1000. Synaptophysin was counterstained with an Alexa fluoro® 488 (green) and nuclear DNA was stained with DAPI (blue).
ab86491 has been referenced in 5 publications.
- Guan YF et al. Anti-depression effects of ketogenic diet are mediated via the restoration of microglial activation and neuronal excitability in the lateral habenula. Brain Behav Immun N/A:N/A (2020). PubMed: 32413556
- Fan Y et al. Up-regulation of TREM2 accelerates the reduction of amyloid deposits and promotes neuronal regeneration in the hippocampus of amyloid beta1-42 injected mice. J Chem Neuroanat 97:71-79 (2019). PubMed: 30790627
- Kim SM et al. TREM2 promotes Aß phagocytosis by upregulating C/EBPa-dependent CD36 expression in microglia. Sci Rep 7:11118 (2017). PubMed: 28894284
- Raha AA et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). WB, IHC ; Mouse . PubMed: 27662313
- Zhang H et al. Depletion of the triggering receptor expressed on myeloid cells 2 inhibits progression of renal cell carcinoma via regulating related protein expression and PTEN-PI3K/Akt pathway. Int J Oncol 49:2498-2506 (2016). PubMed: 27779645