Anti-TREM2 antibody [RM0139-5J46] (ab86491)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-TREM2 antibody [RM0139-5J46]
See all TREM2 primary antibodies -
Description
Rat monoclonal [RM0139-5J46] to TREM2 -
Host species
Rat -
Specificity
This antibody detects mouse TREM2, but not TREM1 in Western blotting. -
Tested applications
Suitable for: ICC/IF, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Mouse recombinant protein of the extracellular EC-domain of TREM2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein A affinity chromatography. -
Clonality
Monoclonal -
Clone number
RM0139-5J46 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab86491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-Fr | 1/250. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 25 kDa. |
Target
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Function
May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. -
Tissue specificity
Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. -
Involvement in disease
Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 54209 Human
- Entrez Gene: 83433 Mouse
- Omim: 605086 Human
- SwissProt: Q9NZC2 Human
- SwissProt: Q99NH8 Mouse
- Unigene: 435295 Human
- Unigene: 261623 Mouse
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Alternative names
- TREM 2 antibody
- TREM-2 antibody
- TREM2 antibody
see all
Images
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![Immunohistochemistry (Frozen sections) - Anti-TREM2 antibody [RM0139-5J46] (ab86491)](https://www.abcam.com/ps/products/86/ab86491/Images/ab86491-202813-anti-trem2-antibody-rm0139-5j46-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-TREM2 antibody [RM0139-5J46] (ab86491)This image is courtesy of an abreview submitted by Dr Ruma Raha-Chowdhury.IHC-Fr image of human blood vessels stained with Ab86491 (1:250). The fixed brain sections were permeabilized using 0.1% PBS-Triton X for 1h. 10% donkey serum was used for 1 hour at 24°C to block protein-protein interactions. The sections were then incubated with Ab8649 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-rat used at 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [RM0139-5J46] (ab86491)](https://www.abcam.com/ps/products/86/ab86491/Images/ab86491-252829-anti-trem2-antibody-rm0139-5j46-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [RM0139-5J46] (ab86491)This image is courtesy of an abreview submitted by Dr Ruma Raha-Chowdhury.Immunofluorescence analysis of 18 day rat embroyonic hypothalmic neurones labelling TREM2 with ab86491. Embryonic hypothalmic neuronal cells were cultured in a neurobasal media with B27 culture suppliment for three weeks and fixed with 2%PFA. A rat monoclonal Alexa fluoro® 568 (red) secondary was used at a concentration of 1/1000. Synaptophysin was counterstained with an Alexa fluoro® 488 (green) and nuclear DNA was stained with DAPI (blue).
Datasheets and documents
References
This product has been referenced in:
- Fan Y et al. Up-regulation of TREM2 accelerates the reduction of amyloid deposits and promotes neuronal regeneration in the hippocampus of amyloid beta1-42 injected mice. J Chem Neuroanat 97:71-79 (2019). Read more (PubMed: 30790627) »
- Kim SM et al. TREM2 promotes Aß phagocytosis by upregulating C/EBPa-dependent CD36 expression in microglia. Sci Rep 7:11118 (2017). Read more (PubMed: 28894284) »
