Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-TREM2 [RM0139-5J46] antibody

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Tissue sections (Blood vessels)
Specification
Blood vessels
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Formaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% TritonX with 0.1x PBS- 10% Donkeys
Dilution
1/250

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluro 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568
Dilution
1/1000

Additional data

Additional Notes
IHC/IF image of human blood vessels stained with Ab86491. The fixed brain section deparafinised and then permeabilise in 0.1% PBS-Triton X for 1h to block non-specific protein-protein interactions. The sections were then incubated with the antibody (Ab86491) used 1/250 dilution, overnight at +4¯C. The secondary antibody was Alexa Fluor­ 568 donkey anti-rat used at a 1/1000 dilution for 1h. TREM2 expressed in the blood vessels and macrophages and colocalised with Ab124834 in red blood cells. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43´M. Very good antibody.

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 06 2013

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