IHC/IF image of human blood vessels stained with Ab86491. The fixed brain section deparafinised and then permeabilise in 0.1% PBS-Triton X for 1h to block non-specific protein-protein interactions. The sections were then incubated with the antibody (Ab86491) used 1/250 dilution, overnight at +4¯C. The secondary antibody was Alexa Fluor 568 donkey anti-rat used at a 1/1000 dilution for 1h. TREM2 expressed in the blood vessels and macrophages and colocalised with Ab124834 in red blood cells. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43´M. Very good antibody.
Dr. Ruma Raha-Chowdhury
Submitted Aug 06 2013
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