Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain section - 50um)
Antigen retrieval step
None
Permeabilization
Yes - PBS-T 3 x 5mn
Specification
Brain section - 50um
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Additional data
Additional Notes
Tissues were fixed with PFA 5% in PBS 1X
Incubation with secondaries: 2 hours
Picture of the dentate gyrus of the adult and healthy WT mouse hippocampus - Iba1 stains microglia
Abcam user community
Verified customer
Submitted Jun 10 2019