The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: 1/10 - 1/50. Acetone fixation of the antigen source is recommended prior to staining.
IHC-P: Use at an assay dependent dilution.
Suitability for use in Western blot and ELISA has not been determined but use in such assays should not necessarily be excluded.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Treponema pallidum is the causative agent of syphilis. It is a gram negative spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella.