Overview

  • Product name
    Anti-Treponema pallidum antibody (HRP)
    See all Treponema pallidum primary antibodies
  • Description
    Rabbit polyclonal to Treponema pallidum (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Specificity
    Recognises all antigens. Non reactive in RPR assay. Has not been absorbed and may react with related microoragisms.
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reactivity testing has not been done. This antibody may crossreact with related organisms.
  • Immunogen

    Highly purified Treponema pallidum.

  • General notes

    Titer: >1:3,000 (indirect FA). Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

    Covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated molar HRP:IgG substitution is 2-3. >95% pure, before conjugation.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Titer: >1:3,000 (indirect FA). Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    Treponema pallidum is the causative agent of syphilis. It is a gram negative spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella.
  • Alternative names
    • T. pallidum antibody

References

ab20095 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab20095que vous avez reçu ne fonctionne pas comme attendu.

Comme convenu j'ai procédé à l'envoi d'une unité de ab20923; le numéro de commande de remplacement gratuit de ce produit est *********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

La publication dont je vous ai parléesur les différentes conditions de démasquage d'antigène est : Emoto et al., J Histochem Cytochem. 2005 Nov ;53 (11) : 1311-21 (Table 2).

N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Answer

Thank you for your enquiry. The IHC testing was done by a customer's laboratory. The antibody was used on paraffin sections after citrate antigen retrieval. They used an amplified colorimetric detection system, but I do not have any additional information about their assay system. Yes, you can use the DAKO HRP detection system. I hope this helps you. If you need additional assistance please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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