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    treslin-antibody-ab124268.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other
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Anti-Treslin antibody (ab124268)

  • Datasheet
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Western blot - Anti-Treslin antibody (ab124268)
  • Western blot - Anti-Treslin antibody (ab124268)

Key features and details

  • Rabbit polyclonal to Treslin
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Treslin antibody
    See all Treslin primary antibodies
  • Description

    Rabbit polyclonal to Treslin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Treslin aa 975-1025.
    Database link: NP_689472.3

  • Positive control

    • 293T whole cell lysate
  • General notes

    Previously labelled as C15orf42. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab124268 was affinity purified using an epitope specific to Treslin immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab124268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 211 kDa.

Target

  • Function

    Regulator of DNA replication and S/M and G2/M checkpoints. Regulates the triggering of DNA replication initiation via its interaction with TOPBP1 by participating to CDK2-mediated loading of CDC45L onto replication origins. Required for the transition from pre-replication complex (pre-RC) to pre-initiation complex (pre-IC). Required to prevent mitotic entry after treatment with ionizing radiation.
  • Sequence similarities

    Belongs to the treslin family.
  • Cellular localization

    Nucleus. Associates with chromatin.
  • Target information above from: UniProt accession Q7Z2Z1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 90381 Human
    • Omim: 613298 Human
    • SwissProt: Q7Z2Z1 Human
    • Unigene: 441708 Human
    • Alternative names

      • C15orf42 antibody
      • chromosome 15 open reading frame 42 antibody
      • replication-stimulating protein antibody
      • SLD3 antibody
      • SLD3 homolog antibody
      • SLD3, yeast , homolog of antibody
      • TICRR antibody
      • TICRR_HUMAN antibody
      • TopBP1-interacting antibody
      • TopBP1-interacting checkpoint and replication regulator antibody
      • Treslin antibody
      see all

    Images

    • Western blot - Anti-Treslin antibody (ab124268)
      Western blot - Anti-Treslin antibody (ab124268)This image is courtesy of an anonymous abreview.
      All lanes : Anti-Treslin antibody (ab124268) at 1/1000 dilution

      Lane 1 : 293 whole cell lysate treated with control siLUC RNAi
      Lanes 2-3 : 293 whole cell lysate treated with RNAi targeting Treslin

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/20000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 211 kDa
      Observed band size: 250 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 130 kDa (possible non-specific binding)


      Exposure time: 2 minutes


      Blocking: 5% milk

    • Western blot - Anti-Treslin antibody (ab124268)
      Western blot - Anti-Treslin antibody (ab124268)
      All lanes : Anti-Treslin antibody (ab124268) at 0.4 µg/ml

      Lane 1 : 293T whole cell lysate at 50 µg
      Lane 2 : 293T whole cell lysate at 15 µg

      Developed using the ECL technique.

      Predicted band size: 211 kDa


      Exposure time: 3 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab124268? Please let us know so that we can cite the reference in this datasheet.

    ab124268 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Treslin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293 cells)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    50 µg
    Treatment
    siRNA for 72h
    Specification
    HEK293 cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted May 15 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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