Anti-Treslin antibody (ab124268)
Key features and details
- Rabbit polyclonal to Treslin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Treslin antibody
See all Treslin primary antibodies -
Description
Rabbit polyclonal to Treslin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Treslin aa 975-1025.
Database link: NP_689472.3 -
Positive control
- 293T whole cell lysate
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General notes
Previously labelled as C15orf42.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab124268 was affinity purified using an epitope specific to Treslin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab124268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2500. Predicted molecular weight: 211 kDa. |
Target
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Function
Regulator of DNA replication and S/M and G2/M checkpoints. Regulates the triggering of DNA replication initiation via its interaction with TOPBP1 by participating to CDK2-mediated loading of CDC45L onto replication origins. Required for the transition from pre-replication complex (pre-RC) to pre-initiation complex (pre-IC). Required to prevent mitotic entry after treatment with ionizing radiation. -
Sequence similarities
Belongs to the treslin family. -
Cellular localization
Nucleus. Associates with chromatin. - Information by UniProt
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Database links
- Entrez Gene: 90381 Human
- Omim: 613298 Human
- SwissProt: Q7Z2Z1 Human
- Unigene: 441708 Human
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Alternative names
- C15orf42 antibody
- chromosome 15 open reading frame 42 antibody
- replication-stimulating protein antibody
see all
Images
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Western blot - Anti-Treslin antibody (ab124268)This image is courtesy of an anonymous abreview.All lanes : Anti-Treslin antibody (ab124268) at 1/1000 dilution
Lane 1 : 293 whole cell lysate treated with control siLUC RNAi
Lanes 2-3 : 293 whole cell lysate treated with RNAi targeting Treslin
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 211 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa (possible non-specific binding)
Exposure time: 2 minutesBlocking: 5% milk
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Western blot - Anti-Treslin antibody (ab124268)All lanes : Anti-Treslin antibody (ab124268) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 211 kDa
Exposure time: 3 minutes
Datasheets and documents
References (0)
ab124268 has not yet been referenced specifically in any publications.
