Anti-Treslin antibody (ab124268)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Treslin antibody
See all Treslin primary antibodies -
Description
Rabbit polyclonal to Treslin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Treslin aa 975-1025.
Database link: NP_689472.3 -
Positive control
- 293T whole cell lysate
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General notes
Previously labelled as C15orf42.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab124268 was affinity purified using an epitope specific to Treslin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2500. Predicted molecular weight: 211 kDa. |
Application notes
Is unsuitable for IP.
Target
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Function
Regulator of DNA replication and S/M and G2/M checkpoints. Regulates the triggering of DNA replication initiation via its interaction with TOPBP1 by participating to CDK2-mediated loading of CDC45L onto replication origins. Required for the transition from pre-replication complex (pre-RC) to pre-initiation complex (pre-IC). Required to prevent mitotic entry after treatment with ionizing radiation. -
Sequence similarities
Belongs to the treslin family. -
Cellular localization
Nucleus. Associates with chromatin. - Information by UniProt
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Database links
- Entrez Gene: 90381 Human
- Omim: 613298 Human
- SwissProt: Q7Z2Z1 Human
- Unigene: 441708 Human
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Alternative names
- C15orf42 antibody
- chromosome 15 open reading frame 42 antibody
- replication-stimulating protein antibody
see all
Images
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Western blot - Anti-Treslin antibody (ab124268)This image is courtesy of an anonymous abreview.All lanes : Anti-Treslin antibody (ab124268) at 1/1000 dilution
Lane 1 : 293 whole cell lysate treated with control siLUC RNAi
Lanes 2-3 : 293 whole cell lysate treated with RNAi targeting Treslin
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 211 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa (possible non-specific binding)
Exposure time: 2 minutesBlocking: 5% milk
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Western blot - Anti-Treslin antibody (ab124268)All lanes : Anti-Treslin antibody (ab124268) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 211 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
References
ab124268 has not yet been referenced specifically in any publications.
