Key features and details
- Rabbit polyclonal to TREX1
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TREX1 antibody
See all TREX1 primary antibodies
DescriptionRabbit polyclonal to TREX1
Tested applicationsSuitable for: IP, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human TREX1 aa 1-270.
Database link: Q9NSU2
- IHC-P: Human small intestine tissue. IP: HeLa whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab238339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
RelevanceTREX1 is the major 3'->5' DNA exonuclease in human cells. The protein is a non processive exonuclease that may serve a proofreading function for a human DNA polymerase. It is also a component of the SET complex, and acts to rapidly degrade 3' ends of nicked DNA during granzyme A mediated cell death. Mutations in this gene result in Aicardi Goutieres syndrome, chilblain lupus, and Cree encephalitis. Multiple transcript variants encoding different isoforms have been found for this gene.
- 3' 5' exonuclease TREX1 antibody
- 3' repair exonuclease 1 antibody
- AGS1 antibody
Paraffin-embedded human small intestine tissue stained for TREX1 using ab238339 at 1/100 dilution in immunohistochemical analysis.
TREX1 was immunoprecipitated from 0.5 mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract with ab238339 at 1/200 dilution.
Lane 1: Control rabbit IgG IP (1 μg) in HeLa whole cell lysate.
Lane 2: ab238339 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 μg (Input).
For Western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at 1/50,000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238339 has not yet been referenced specifically in any publications.