Anti-TREX1 antibody (ab83890)
Key features and details
- Rabbit polyclonal to TREX1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TREX1 antibody
See all TREX1 primary antibodies -
Description
Rabbit polyclonal to TREX1 -
Host species
Rabbit -
Specificity
This antibody will not cross-react with the related protein TREX2. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The mouse cross reactivity is lot specific. Please contact technical team for more information.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 39 kDa).
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IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 39 kDa). |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Relevance
TREX1 is the major 3'->5' DNA exonuclease in human cells. The protein is a non processive exonuclease that may serve a proofreading function for a human DNA polymerase. It is also a component of the SET complex, and acts to rapidly degrade 3' ends of nicked DNA during granzyme A mediated cell death. Mutations in this gene result in Aicardi Goutieres syndrome, chilblain lupus, and Cree encephalitis. Multiple transcript variants encoding different isoforms have been found for this gene. -
Cellular localization
Cytoplasmic, Endoplasmic reticulum and Nuclear -
Database links
- Entrez Gene: 11277 Human
- Entrez Gene: 22040 Mouse
- Omim: 606609 Human
- SwissProt: Q9NSU2 Human
- SwissProt: Q91XB0 Mouse
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Alternative names
- 3' 5' exonuclease TREX1 antibody
- 3' repair exonuclease 1 antibody
- AGS1 antibody
see all
Images
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Lane 1 : Anti-TREX1 antibody (ab83890) at 0.5 µg/ml
Lane 2 : Anti-TREX1 antibody (ab83890) at 1 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit secondary
Predicted band size: 39 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted? -
ab83890 at 2.5µg/ml staining TREX1 in Human spleen tissue.
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Immunofluorescence of TREX1 in Human Spleen cells using ab83890 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab83890 has been referenced in 2 publications.
- Lombardo KA et al. BCG invokes superior STING-mediated innate immune response over radiotherapy in a carcinogen murine model of urothelial cancer. J Pathol 256:223-234 (2022). PubMed: 34731491
- Prati B et al. Three Prime Repair Exonuclease 1 (TREX1) expression correlates with cervical cancer cells growth in vitro and disease progression in vivo. Sci Rep 9:351 (2019). PubMed: 30674977