Overview

  • Product name

    Anti-TREX1 antibody [EPR14985]
    See all TREX1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14985] to TREX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TREX1 aa 200-350. The exact sequence is proprietary.
    Database link: Q9NSU2

  • Positive control

    • Raji, Daudi and HeLa whole cell lysate (ab150035). Human colon and adenocarcinoma of colon tissue. HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/50 - 1/100.

Target

  • Relevance

    TREX1 is the major 3'->5' DNA exonuclease in human cells. The protein is a non processive exonuclease that may serve a proofreading function for a human DNA polymerase. It is also a component of the SET complex, and acts to rapidly degrade 3' ends of nicked DNA during granzyme A mediated cell death. Mutations in this gene result in Aicardi Goutieres syndrome, chilblain lupus, and Cree encephalitis. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • 3' 5' exonuclease TREX1 antibody
    • 3' repair exonuclease 1 antibody
    • AGS1 antibody
    • AGS5 antibody
    • CRV antibody
    • Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody
    • DKFZp434J0310 antibody
    • DNase III antibody
    • DRN3 antibody
    • HERNS antibody
    • Three prime repair exonuclease 1 antibody
    • TREX1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human colon adenocarcinoma tissue sections labeling TREX1 using ab185228 at a 1/100 dilution. Hematoxylin counterstain.

  • Immunofluorescent analysis of -20°C Acetone fixed HeLa cells labeling TREX1 using ab185228 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin embedded Human colon tissue sections labeling TREX1 using ab185228 at a 1/100 dilution. Hematoxylin counterstain.

  • All lanes : Anti-TREX1 antibody [EPR14985] (ab185228) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

References

This product has been referenced in:

  • Prati B  et al. Three Prime Repair Exonuclease 1 (TREX1) expression correlates with cervical cancer cells growth in vitro and disease progression in vivo. Sci Rep 9:351 (2019). Read more (PubMed: 30674977) »
  • Li P  et al. Aicardi-Goutières syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion. Nucleic Acids Res 45:4619-4631 (2017). Read more (PubMed: 28334850) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (T cells)
Permeabilization
Yes - Fix&Perm Kit (Life Technologies)
Gating Strategy
CD4+ T cells
Specification
T cells
Preparation
Cell harvesting/tissue preparation method: CD4+ T cells from human PBMCs
Sample buffer: used with Fix&Perm Kit (Life Technologies)

Radiana Trifonova

Verified customer

Submitted May 05 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up