• Product name
    Anti-TRF1 antibody [3H11]
    See all TRF1 primary antibodies
  • Description
    Mouse monoclonal [3H11] to TRF1
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IF, ELISpotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human TRF1 protein.

  • Positive control
    • HeLa cell nuclear lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab14397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/100 - 1/200. Predicted molecular weight: 50 kDa.
ICC/IF Use a concentration of 1 µg/ml.
ELISpot Use 1µg for 106 cells.


  • Function
    Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • Tissue specificity
    Highly expressed and ubiquitous. Isoform Pin2 predominates.
  • Sequence similarities
    Contains 1 HTH myb-type DNA-binding domain.
  • Domain
    The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • Post-translational
    Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • hTRF1 AS antibody
    • NIMA interacting protein 2 antibody
    • NIMA-interacting protein 2 antibody
    • PIN 2 antibody
    • PIN2 antibody
    • t TRF1 antibody
    • Telomeric protein Pin2 antibody
    • Telomeric protein Pin2/TRF1 antibody
    • Telomeric repeat binding factor (NIMA interacting) 1 antibody
    • Telomeric repeat binding factor 1 antibody
    • Telomeric repeat binding protein 1 antibody
    • Telomeric repeat-binding factor 1 antibody
    • TERF 1 antibody
    • Terf1 antibody
    • TERF1_HUMAN antibody
    • TRBF 1 antibody
    • TRBF1 antibody
    • TRF 1 antibody
    • TRF antibody
    • TTAGGG repeat binding factor 1 antibody
    • TTAGGG repeat-binding factor 1 antibody
    see all


  • Anti-TRF1 antibody [3H11] (ab14397) at 1/200 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 50 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab14397 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14397, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab14397 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14397, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


ab14397 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (normal human fibroblasts)
Loading amount
60 µg
normal human fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted May 08 2006


Thank you for your enquiry. I am sorry to hear that you have been having difficulties with mouse monoclonal [3H11] to TRF1 (ab14397). I have reas teh details that you have submitted and I have a few comments. Please can you tell me whether you are detecting any form of endogenous TRF1 in your human fibroblasts samples? If you are not detecting either the endogenous or your overexpressed TRF1 protein I would like to suggest that you reduce the dilution of the antibody to ~1:100. The on line datasheet recommends that the antibody is applied at a dilution of 1/100 - 1/200. I would also like to recommend that an overnight incubation at 4oC is perfomed using 3% BSA (as opposed to non-fat dried milk). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry. The predicted molecular weight of human TRF1 is approximately 50 kDa. There was a mistake on the online datasheet for ab10579 and I have updated it. Thank you for bringing this to our attention and please contact us again if you have any additional questions.

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Thank you for your reply, I appreciate your opinion on this matter and in light of the further information you sent me I agree that if you detected TRF1 without a problem you should see TRF2 too. Would you like to try a replacement vial or prefer a refund or credit note? I look forward to hearing from you to arrange this,

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BATCH NUMBER 77744 ORDER NUMBER PR340939 DESCRIPTION OF THE PROBLEM WB on human cell lysates expressing a mammalian expression vector encoding human TRF1 tagged with a flag epitope tag. The immunodetection of flag-TRF1 protein could be seen on immunoblots using anti-flag antibody (Sigma) but no specific band at the expected molecular weigth was observed when the menbrane was hybridised with Ab14397. SAMPLE human fibroblasts cell lysates stably expressing a mammalian expression vector encoding human TRF1 tagged with a flag epitope tag. PRIMARY ANTIBODY Ab14397 dilution 1/250 in (TBS-T) 3 hr at room temperature and O/N at 4?C membrane washed with TBS-T 0,05% 3 times for 5 min at room temperature DETECTION METHOD enhanced chemiluminescence was used (Cat NEL105 ; Amersham) product currently used in the lab POSITIVE AND NEGATIVE CONTROLS USED A tagged TRF1 protein was overexpressed in the test ANTIBODY STORAGE CONDITIONS Antibody arrived in solution and was used immediately after delivery on Friday the 10th of February. Now, the antibody is aliquoted and stored at -20?C SAMPLE PREPARATION lysis buffer used (condition optimized for the detection of another telomeric protein TRF2) 50 mM tris-HCl, pH 7,4 ; 1% NP40 ; 0,25% sodium deoxycholate; 150 mM NaCl ; 1 mM EGTA ; 1 mM PMSF ; 1microgram/ml of leupeptin, aprotinin ; pepstatin; 1 mM Na3Vo4 ; 1 mM NaF boiled samples AMOUNT OF PROTEIN LOADED 60 microgram of total cell lysates ELECTROPHORESIS/GEL CONDITIONS 8% polyacrylamide gel and reducing condition TRANSFER AND BLOCKING CONDITIONS PVDF membrane was used blocking inTBS containing 5% nonfat dry milk and 0,1% Tween (TBS-T) 20 for 45 min at room temperature SECONDARY ANTIBODY sheep anti-mouse HRP conjugated IgG ( [a competitor], product currently used in the lab) dilution 1/5000 in (TBS-T), 1h at room membrane washed with TBS-T 0,05% 3 times for 5 min at room temperature HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1

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Many thanks for taking the time to provide all your protocol information, it is very useful to understand your problem and I'm sorry to hear you are experiencing unsatisfactory results with ab14397. I think your protocol is very good and the fact that your flag tag is detected is very positive; however, I am wondering if you are not expressing the whole sequence of the protein and as it is a monoclonal antibody it will only recognize one single epitope. I would therefore recommend to run a positive control along your samples, we recommend HeLa nuclear lysate. I was able to find out that the antibody was used at 1:100-1:200 so your choice of 1:250 is only a little above that dilution and should be ok. Please let me know your thoughts on the matter of the protein sequence, I look forward to hearing from you soon,

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