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Thank you for your prompt reply and your suggestions. The TRF1 sequence used is the complete human sequence and we have run alongside normal human fibroblasts cells and the endogenous TRF1 protein was not detected either. OK, as you suggest we could used Hela nuclear extracts as positive control. I would like to mention that with our conditions we nicely detected the expression of the endogenous TRF2, another telomeric protein therefore I don't understand why we failed to detect TRF1 under the conditions used. Thank you for your assistance
Asked on Feb 16 2006
Thank you for your reply, I appreciate your opinion on this matter and in light of the further information you sent me I agree that if you detected TRF1 without a problem you should see TRF2 too. Would you like to try a replacement vial or prefer a refund or credit note? I look forward to hearing from you to arrange this,
Answered on Feb 17 2006