Key features and details
- Rabbit polyclonal to TRF1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRF1 antibody
See all TRF1 primary antibodies
DescriptionRabbit polyclonal to TRF1
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chinese hamster
Recombinant fragment corresponding to Human TRF1 aa 260-439.
AAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKSVSDKQSAVTESSE GTVSLLRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATE SRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLH YKFNNRTSVMLKDRWRTMKKLKLISSDSED
Database link: P54274
- IHC-P: Human skeletal muscle tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab236325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
FunctionBinds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Tissue specificityHighly expressed and ubiquitous. Isoform Pin2 predominates.
Sequence similaritiesContains 1 HTH myb-type DNA-binding domain.
DomainThe acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
The TRFH dimerization region mediates the interaction with TINF2.
modificationsPhosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
- Information by UniProt
- hTRF1 AS antibody
- NIMA interacting protein 2 antibody
- NIMA-interacting protein 2 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236325 has not yet been referenced specifically in any publications.