Overview

  • Product name

    Anti-TRF1 antibody - ChIP Grade
    See all TRF1 primary antibodies
  • Description

    Rabbit polyclonal to TRF1 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Human full length recombinant protein expressed in bacteria.

  • Positive control

    • HL60 WCE lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab1423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution. PubMed: 1946086
WB 1/2000. Detects a band of approximately 60-65 kDa. An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended.
ICC/IF Use at an assay dependent dilution. PubMed: 20427319
IP Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
    • Tissue specificity

      Highly expressed and ubiquitous. Isoform Pin2 predominates.
    • Sequence similarities

      Contains 1 HTH myb-type DNA-binding domain.
    • Domain

      The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
      The TRFH dimerization region mediates the interaction with TINF2.
    • Post-translational
      modifications

      Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
      ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
      Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
    • Information by UniProt
    • Database links

    • Alternative names

      • hTRF1 AS antibody
      • NIMA interacting protein 2 antibody
      • NIMA-interacting protein 2 antibody
      • PIN 2 antibody
      • PIN2 antibody
      • t TRF1 antibody
      • Telomeric protein Pin2 antibody
      • Telomeric protein Pin2/TRF1 antibody
      • Telomeric repeat binding factor (NIMA interacting) 1 antibody
      • Telomeric repeat binding factor 1 antibody
      • Telomeric repeat binding protein 1 antibody
      • Telomeric repeat-binding factor 1 antibody
      • TERF 1 antibody
      • Terf1 antibody
      • TERF1_HUMAN antibody
      • TRBF 1 antibody
      • TRBF1 antibody
      • TRF 1 antibody
      • TRF antibody
      • TTAGGG repeat binding factor 1 antibody
      • TTAGGG repeat-binding factor 1 antibody
      see all

    Images

    • Western blot using anti-TRF1 at 1/2000. A single band at 60-65 kD is detected. Western blot using anti-TRF1 at 1/2000. A single band at 60-65 kD is detected.
    • Cells transfected with a TRF1-GFP contruct. On the left: immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre: fluorescence from the GFP. Right: merge of both images showing overlap of GFP fluorescence and antibody staining.

    • The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).

    References

    This product has been referenced in:

    • Ge J  et al. SIRT6 participates in the quality control of aged oocytes via modulating telomere function. Aging (Albany NY) 11:1965-1976 (2019). ICC/IF ; Mouse . Read more (PubMed: 30926765) »
    • Margalef P  et al. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell 172:439-453.e14 (2018). Mouse . Read more (PubMed: 29290468) »
    See all 31 Publications for this product

    Customer reviews and Q&As

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    1-4 of 4 Abreviews

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (8% resolution gel)
    Sample
    Human Cell lysate - whole cell (hepatocellular carcinoma)
    Specification
    hepatocellular carcinoma
    Treatment
    Inhibition of one gene's expression using lentiviral shRNA
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Jul 16 2013

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Breast carcinoma)
    Specification
    Breast carcinoma
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X-100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Aug 23 2009

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cell)
    Loading amount
    100 µg
    Specification
    HeLa cell
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%

    Dr. Geun Hyoung Ha

    Verified customer

    Submitted May 27 2009

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela CELLS)
    Loading amount
    50 µg
    Specification
    Hela CELLS
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Geun-Hyoung Ha

    Verified customer

    Submitted Apr 30 2009

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