• Product name
    Anti-TRF1 antibody [TRF-78]
    See all TRF1 primary antibodies
  • Description
    Mouse monoclonal [TRF-78] to TRF1
  • Host species
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human TRF1 protein produce in baculovirus



Our Abpromise guarantee covers the use of ab10579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 50 kDa.
IHC-P 1/100. PubMed: 18641004
ICC/IF 1/100.


  • Function
    Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • Tissue specificity
    Highly expressed and ubiquitous. Isoform Pin2 predominates.
  • Sequence similarities
    Contains 1 HTH myb-type DNA-binding domain.
  • Domain
    The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • Post-translational
    Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • hTRF1 AS antibody
    • NIMA interacting protein 2 antibody
    • NIMA-interacting protein 2 antibody
    • PIN 2 antibody
    • PIN2 antibody
    • t TRF1 antibody
    • Telomeric protein Pin2 antibody
    • Telomeric protein Pin2/TRF1 antibody
    • Telomeric repeat binding factor (NIMA interacting) 1 antibody
    • Telomeric repeat binding factor 1 antibody
    • Telomeric repeat binding protein 1 antibody
    • Telomeric repeat-binding factor 1 antibody
    • TERF 1 antibody
    • Terf1 antibody
    • TERF1_HUMAN antibody
    • TRBF 1 antibody
    • TRBF1 antibody
    • TRF 1 antibody
    • TRF antibody
    • TTAGGG repeat binding factor 1 antibody
    • TTAGGG repeat-binding factor 1 antibody
    see all


  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling TRF1 with ab10579. cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Anti-TRF1 antibody [TRF-78] (ab10579).  The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • All lanes : Anti-TRF1 antibody [TRF-78] (ab10579) at 4 µg/ml

    Lane 1 : HeLa Nuclear Cell lysate
    Lane 2 : HeLa Cell lysate
    Lane 3 : HEK-293T Cell lysate
    Lane 4 : U2OS Cell lysate
    Lane 5 : HepG2 Cell lysate

    All lanes : Goat Anti-Mouse IgG-Peroxidase

    Predicted band size: 50 kDa

  • Immunofluorescent imaging of human cells (U2OS) with ab10579 confirms the specificity of this antibody.  A few intense nuclear foci are seen in interphase cells, corresponding to telomeric localisation.  The complete absence of background nuclear or cytoplasmic staining confirms the specificity of this antibody. This image is in exact agreement with numerous published reports.

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees. Nuclei are visualised using Hoechst stain. 

  • ab10579 at 1/500 staining human HeLa cells by ICC/IF. The cells were parafomaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 555 conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview


This product has been referenced in:
  • Wang J  et al. Photosensitization of A2E triggers telomere dysfunction and accelerates retinal pigment epithelium senescence. Cell Death Dis 9:178 (2018). ICC/IF ; Human . Read more (PubMed: 29415988) »
  • Krastev DB  et al. Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets. Nat Commun 9:2016 (2018). Read more (PubMed: 29789535) »

See all 35 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (Brain)
Yes - Triton Tx 0,3%
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Dr. Milan Stojiljkovic

Verified customer

Submitted Mar 10 2016

Western blot
Mouse Cell lysate - whole cell (HEK293T-TRF1 HEK293T-TRF2)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Blocking step
(agent) for 1 hour(s) and 10 minute(s) · Concentration: 2% · Temperature: 37°C

Giovanna Roncador

Verified customer

Submitted Jul 24 2015

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Mouse Cell (oocyte)
Yes - 0.01% triton-X100

Abcam user community

Verified customer

Submitted Apr 28 2014

Merci de nous avoir contactés.

En compensation des 4 unités de ab10579 qui n'ont pas fonctionné, j'ai mis en place l'envoi d'une unité de ab32084 et d'une unité de ab1838, les numéros de commandes sont...

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Thank you for your reply.

I hope the new vial will provide good results!

I am sorry that I have not seen the crossreactivity note on the datasheet of the secondary antibody.I might would nevertheless performa control slide using you...

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Thank you very much for having provided all these additional information.

I have seen the results and I agree that the staining is not as the previous staining. As for the reasons explained before, I just would like to ask you to confirm the ...

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Thank you for contacting us.

While to our knowledgethis TRF1 antibody has not been tested for cross-reactivity with TRF2, it is not likely to detect that protein. The homology between TRF1 and TRF2 is only around 17%. We have not had any cust...

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Thank you for contacting us.

At this time we have not determined the light chain type of this antibody, we apologize for any inconvenience.

I hope this information is helpful to you. Please do not hesitate to contact us if you...

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Thank you for contacting us.

For the antibodies with the clone numbers you mentioned we have the follwoing immunogen information:

BED5 57-6 - ab47717 (Human full length recombinant protein expressed in bacteria)

BED5 - ab...

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Thank you for your email.

Your credit note ID is xxx for ab10579.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, pl...

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