Overview

  • Product name

    Anti-TRF1 antibody [TRF-78]
    See all TRF1 primary antibodies
  • Description

    Mouse monoclonal [TRF-78] to TRF1
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human TRF1 protein produce in baculovirus

  • Positive control

    • ICC/IF: HeLa cells. WB: HeLa, HEK-293, HepG2 and U-2 OS cell lysate. HeLa nuclear cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab10579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 50 kDa.
IHC-P 1/100. PubMed: 18641004
ICC/IF 1/100.

Target

  • Function

    Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • Tissue specificity

    Highly expressed and ubiquitous. Isoform Pin2 predominates.
  • Sequence similarities

    Contains 1 HTH myb-type DNA-binding domain.
  • Domain

    The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • Post-translational
    modifications

    Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt
  • Database links

  • Alternative names

    • hTRF1 AS antibody
    • NIMA interacting protein 2 antibody
    • NIMA-interacting protein 2 antibody
    • PIN 2 antibody
    • PIN2 antibody
    • t TRF1 antibody
    • Telomeric protein Pin2 antibody
    • Telomeric protein Pin2/TRF1 antibody
    • Telomeric repeat binding factor (NIMA interacting) 1 antibody
    • Telomeric repeat binding factor 1 antibody
    • Telomeric repeat binding protein 1 antibody
    • Telomeric repeat-binding factor 1 antibody
    • TERF 1 antibody
    • Terf1 antibody
    • TERF1_HUMAN antibody
    • TRBF 1 antibody
    • TRBF1 antibody
    • TRF 1 antibody
    • TRF antibody
    • TTAGGG repeat binding factor 1 antibody
    • TTAGGG repeat-binding factor 1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling TRF1 with ab10579. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Anti-TRF1 antibody [TRF-78] (ab10579).  The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • All lanes : Anti-TRF1 antibody [TRF-78] (ab10579) at 1/1000 dilution

    Lane 1 : SAOS2 (Human osteosarcoma cell line) cell lysate
    Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti Mouse polyclonal IRDye 800CW at 1/1000 dilution

    Predicted band size: 50 kDa


    Exposure time: 5 minutes


    Blocking step
    Milk as blocking agent for 2 hours · Concentration: 5% · Temperature: 21°C.
    Incubation time
    12 hours · Temperature: 4°C · Diluent: 5% milk in TBST.
  • All lanes : Anti-TRF1 antibody [TRF-78] (ab10579) at 4 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 4 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

    Predicted band size: 50 kDa

  • Immunofluorescent imaging of human cells (U2OS) with ab10579 confirms the specificity of this antibody.  A few intense nuclear foci are seen in interphase cells, corresponding to telomeric localisation.  The complete absence of background nuclear or cytoplasmic staining confirms the specificity of this antibody. This image is in exact agreement with numerous published reports.

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees. Nuclei are visualised using Hoechst stain. 

  • ab10579 at 1/500 staining human HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF. The cells were parafomaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 555 conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:

  • Wallace HA  et al. Condensin II subunit NCAPH2 associates with shelterin protein TRF1 and is required for telomere stability. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 31026066) »
  • Wang L  et al. Dual roles of TRF1 in tethering telomeres to the nuclear envelope and protecting them from fusion during meiosis. Cell Death Differ 25:1174-1188 (2018). Read more (PubMed: 29311622) »
See all 43 Publications for this product

Customer reviews and Q&As

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1-9 of 9 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MC3T3)
Permeabilization
Yes - NP40
Specification
MC3T3
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 05 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS and SAOS-2)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
U2OS and SAOS-2
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Feb 19 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 11 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain)
Permeabilization
Yes - Triton Tx 0,3%
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Dr. Milan Stojiljkovic

Verified customer

Submitted Mar 10 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293T-TRF1 HEK293T-TRF2)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
HEK293T-TRF1 HEK293T-TRF2
Blocking step
(agent) for 1 hour(s) and 10 minute(s) · Concentration: 2% · Temperature: 37°C

Giovanna Roncador

Verified customer

Submitted Jul 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Mouse Cell (oocyte)
Specification
oocyte
Permeabilization
Yes - 0.01% triton-X100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 28 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (B lymphocyte)
Specification
B lymphocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 29 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Lung)
Specification
Lung
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Aug 23 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 0.5%

Abcam user community

Verified customer

Submitted Mar 29 2007

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