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I am seeing a band at 70 kDa with ab10579 against TRF1. The band size should be 50 kDa or 56 kDa if there are post-translational modification. I am seeing the correct size band with the TRF2 antibody ab13579. Is there any explanation for the 70 kDa band for TRF1?
Asked on Mar 22 2006
Thank you for your enquiry and your patience. There is no explanation for a band only observed at 70 kDa and we have not observed this band previously. I contacted the originator of the antibody and they have also not observed this band. Could you please fill out the questionnaire at the following link so that might be able to better assist you? https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=10579&mode=questionaire Can you also send me a picture of your blot? An image and a protocol could be very helpful. I look forward to your reply.
Answered on Mar 24 2006