Anti-TRF2 antibody [4A794] (ab13579)
Key features and details
- Mouse monoclonal [4A794] to TRF2
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TRF2 antibody [4A794]
See all TRF2 primary antibodies -
Description
Mouse monoclonal [4A794] to TRF2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human TRF2. Baculovirus expressing full length TRF2 protein. NP_005643
Database link: Q15554 -
Positive control
- WB: Jurkat and HeLa cell lysate Flow Cyt (Intra): Jurkat and HeLa cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4A794 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 4 µg/ml. Detects a band of approximately 66 kDa.
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 4 µg/ml. Detects a band of approximately 66 kDa. |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. -
Tissue specificity
Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes. -
Sequence similarities
Contains 1 HTH myb-type DNA-binding domain. -
Domain
The TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase. - Information by UniProt
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Database links
- Entrez Gene: 7014 Human
- Omim: 602027 Human
- SwissProt: Q15554 Human
- Unigene: 63335 Human
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Alternative names
- Telomeric DNA binding protein antibody
- Telomeric DNA-binding protein antibody
- Telomeric repeat binding factor 2 antibody
see all
Images
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ab13579 staining TRF2 in human bladder carcinoma cells using Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigeen retrieval carried out by heat treatment in Citrate buffer pH6.0. Primary antibody diluted to 4μg/ml.
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Anti-TRF2 antibody [4A794] (ab13579) at 2 µg/ml + Jurkat cell lysate
Secondary
Goat anti-mouse Ig HRP -
ab13579 (1µg/ml) staining TRF2 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the germinal follicle.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Anti-TRF2 antibody [4A794] (ab13579) + HeLa cell lysate
Performed under reducing conditions. -
Overlay histogram showing HeLA cells stained with ab13579 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13579, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Flow Cytometry (Intracellular) analysis of Jurkat cells labeling RF2 with ab13579 (red). The shaded histogram represents cells alone and blue represents isotype control.
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Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.
Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab13579 has been referenced in 41 publications.
- Travina AO et al. The Long Linker Region of Telomere-Binding Protein TRF2 Is Responsible for Interactions with Lamins. Int J Mol Sci 22:N/A (2021). PubMed: 33804854
- Lezaja A et al. RPA shields inherited DNA lesions for post-mitotic DNA synthesis. Nat Commun 12:3827 (2021). PubMed: 34158486
- Kychygina A et al. Progerin impairs 3D genome organization and induces fragile telomeres by limiting the dNTP pools. Sci Rep 11:13195 (2021). PubMed: 34162976
- Sharma S et al. Human telomerase is directly regulated by non-telomeric TRF2-G-quadruplex interaction. Cell Rep 35:109154 (2021). PubMed: 34010660
- Savelyev NV et al. PARP1 Regulates the Biogenesis and Activity of Telomerase Complex Through Modification of H/ACA-Proteins. Front Cell Dev Biol 9:621134 (2021). PubMed: 34095104