• Product name
    Anti-TRF2 antibody [4A794] - ChIP Grade
    See all TRF2 primary antibodies
  • Description
    Mouse monoclonal [4A794] to TRF2 - ChIP Grade
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ICC, IP, ChIP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Indian muntjac
  • Immunogen

    Baculovirus expressing full length TRF2 protein.

  • Positive control
    • Jurkat cell lysate (WB)



Our Abpromise guarantee covers the use of ab13579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 4 µg/ml. Detects a band of approximately 66 kDa.
ICC Use at an assay dependent concentration.
IP Use 2µg for 106 cells.
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20679250
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Function
    Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
  • Tissue specificity
    Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
  • Sequence similarities
    Contains 1 HTH myb-type DNA-binding domain.
  • Domain
    The TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Telomeric DNA binding protein antibody
    • Telomeric DNA-binding protein antibody
    • Telomeric repeat binding factor 2 antibody
    • Telomeric repeat binding protein 2 antibody
    • Telomeric repeat-binding factor 2 antibody
    • TERF 2 antibody
    • Terf2 antibody
    • TERF2_HUMAN antibody
    • TRBF 2 antibody
    • TRBF2 antibody
    • TRF 2 antibody
    • TRF2 antibody
    • TTAGGG repeat binding factor 2 antibody
    • TTAGGG repeat-binding factor 2 antibody
    see all


  • ab13579 staining TRF2 in HeLa cells by Immunocytochemistry/ Immunofluorescence.
    The cells were washed in PBS, fixed for 15 minutes at room temperature in 4% formaldehyde in PBS, and then washed for 5 minutes with PBS. The cells were then dehydrated with cold methanol at room temperature for 10 minutes, washed, and blocked with blocking solution (3% skim milk, 0.05% Tween-20 in PBS) at room temperature for 1 hour. ab13579 added at 2 µg/ml at 37?°C for 1 hour. After washing three times, 5 µg/mL fluorescein (TRITC) goat anti-mouse (Abcam) as the secondary antibody in blocking solution was added and incubated at room temperature for 1 hour.
  • ab13579 staining TRF2 in bladder carcinoma cells using Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigeen retrieval carried out by heat treatment in Citrate buffer pH6.0. Primary antibody diluted to 4μg/ml.

  • Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579.  2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.

    Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.
  • ab13579 (1µg/ml) staining TRF2 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the germinal follicle.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing HeLA cells stained with ab13579 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13579, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Ling X  et al. TERT regulates telomere-related senescence and apoptosis through DNA damage response in male germ cells exposed to BPDE in vitro and to B[a]P in vivo. Environ Pollut 235:836-849 (2018). Mouse . Read more (PubMed: 29353801) »
  • Yuan F  et al. Nucleolar TRF2 attenuated nucleolus stress-induced HCC cell-cycle arrest by altering rRNA synthesis. Cell Death Dis 9:518 (2018). Read more (PubMed: 29725012) »
See all 26 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Jurkat cells)
Loading amount
200000 cells
Jurkat cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Submitted Nov 25 2009


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