Recombinant
RabMAb

Recombinant Anti-TRF2 antibody [EPR3517(2)] (ab108997)

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Overview

  • Product name

    Anti-TRF2 antibody [EPR3517(2)]
    See all TRF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3517(2)] to TRF2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human TRF2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: HT-29, HeLa, and Jurkat whole cell lysate (ab7899); ICC/IF: HeLa and Jurkat cells. IHC-P: Human tonsil and spleen tissue. FC: HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab108997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.
Flow Cyt 1/300.
ICC/IF 1/300.

For unpurified use at 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

    • Tissue specificity

      Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.

    • Sequence similarities

      Contains 1 HTH myb-type DNA-binding domain.

    • Domain

      The TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
    • Information by UniProt

    • Database links

      • Alternative names

        • Telomeric DNA binding protein antibody
        • Telomeric DNA-binding protein antibody
        • Telomeric repeat binding factor 2 antibody
        • Telomeric repeat binding protein 2 antibody
        • Telomeric repeat-binding factor 2 antibody
        • TERF 2 antibody
        • Terf2 antibody
        • TERF2_HUMAN antibody
        • TRBF 2 antibody
        • TRBF2 antibody
        • TRF 2 antibody
        • TRF2 antibody
        • TTAGGG repeat binding factor 2 antibody
        • TTAGGG repeat-binding factor 2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TRF2 with Purified ab108997 at 1:1000 dilution (3.4 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
        Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

        Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TRF2 with Purified ab108997 at 1:300 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      • Flow Cytometry - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
        Flow Cytometry - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

        Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRF2 with Purified ab108997 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      • Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
        Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
      • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
        Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

        ab108997 at 1/100 dilution staining TRF2 in HeLa cells by Immunofluorescence.

        This image was generated using the unpurified version of the product. 

         

      • Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
        Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
        All lanes : Anti-TRF2 antibody [EPR3517(2)] (ab108997) at 1/1000 dilution

        Lane 1 : HT29 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 56 kDa



        This image was generated using the unpurified version of the product. 

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

        ab108997 at 1/250 dilution staining TRF2 in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

        This image was generated using the unpurified version of the product. 

         

      References

      This product has been referenced in:

      • Kulak O  et al. Disruption of Wnt/ß-Catenin Signaling and Telomeric Shortening Are Inextricable Consequences of Tankyrase Inhibition in Human Cells. Mol Cell Biol 35:2425-35 (2015). Read more (PubMed: 25939383) »
      • Tang M  et al. Disease mutant analysis identifies a new function of DAXX in telomerase regulation and telomere maintenance. J Cell Sci 128:331-41 (2015). ICC/IF . Read more (PubMed: 25416818) »
      See all 4 Publications for this product

      Publishing research using ab108997? Please let us know so that we can cite the reference in this datasheet.

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