Recombinant Anti-TRF2 antibody [EPR3517(2)] (ab108997)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3517(2)] to TRF2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TRF2 antibody [EPR3517(2)]
See all TRF2 primary antibodies -
Description
Rabbit monoclonal [EPR3517(2)] to TRF2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HT-29, HeLa, and Jurkat whole cell lysate (ab7899); ICC/IF: HeLa and Jurkat cells. IHC-P: Human tonsil and spleen tissue. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3517(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/300.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
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ICC/IF |
1/300.
For unpurified use at 1/100 - 1/250. |
Notes |
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Flow Cyt (Intra)
1/300. |
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
ICC/IF
1/300. For unpurified use at 1/100 - 1/250. |
Target
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Function
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. -
Tissue specificity
Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes. -
Sequence similarities
Contains 1 HTH myb-type DNA-binding domain. -
Domain
The TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase. - Information by UniProt
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Database links
- Entrez Gene: 7014 Human
- Omim: 602027 Human
- SwissProt: Q15554 Human
- Unigene: 63335 Human
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Alternative names
- Telomeric DNA binding protein antibody
- Telomeric DNA-binding protein antibody
- Telomeric repeat binding factor 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TRF2 with Purified ab108997 at 1:1000 dilution (3.4 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TRF2 with Purified ab108997 at 1:300 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRF2 with Purified ab108997 at 1/300 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Anti-TRF2 antibody [EPR3517(2)] (ab108997) at 1/1000 dilution + HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5%NFDM/TBST
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ab108997 at 1/100 dilution staining TRF2 in HeLa cells by Immunofluorescence.
This image was generated using the unpurified version of the product.
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All lanes : Anti-TRF2 antibody [EPR3517(2)] (ab108997) at 1/1000 dilution
Lane 1 : HT29 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDaThis image was generated using the unpurified version of the product.
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ab108997 at 1/250 dilution staining TRF2 in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.
This image was generated using the unpurified version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab108997 has been referenced in 12 publications.
- Betlej G et al. RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines. J Bone Oncol 36:100448 (2022). PubMed: 35942470
- Maciejewska N et al. Teloxantron inhibits the processivity of telomerase with preferential DNA damage on telomeres. Cell Death Dis 13:1005 (2022). PubMed: 36437244
- Yang L et al. TAZ maintains telomere length in TNBC cells by mediating Rad51C expression. Breast Cancer Res 23:89 (2021). PubMed: 34488828
- Victor J et al. SARS-CoV-2 triggers DNA damage response in Vero E6 cells. Biochem Biophys Res Commun 579:141-145 (2021). PubMed: 34600299
- Zhang Y et al. 6-Dithio-2'-deoxyguanosine analogs induce reactive oxygen species-mediated tumor cell apoptosis via bi-targeting thioredoxin 1 and telomerase. Toxicol Appl Pharmacol 401:115079 (2020). PubMed: 32497534