Overview

  • Product name

    Anti-TRF2 antibody - N-terminal
    See all TRF2 primary antibodies
  • Description

    Rabbit polyclonal to TRF2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TRF2 (N terminal). 17 amino acid peptide.
    Database link: Q15554

  • Positive control

    • 293 cell lysate; human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab191704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 59 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
  • Tissue specificity

    Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
  • Sequence similarities

    Contains 1 HTH myb-type DNA-binding domain.
  • Domain

    The TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • Telomeric DNA binding protein antibody
    • Telomeric DNA-binding protein antibody
    • Telomeric repeat binding factor 2 antibody
    • Telomeric repeat binding protein 2 antibody
    • Telomeric repeat-binding factor 2 antibody
    • TERF 2 antibody
    • Terf2 antibody
    • TERF2_HUMAN antibody
    • TRBF 2 antibody
    • TRBF2 antibody
    • TRF 2 antibody
    • TRF2 antibody
    • TTAGGG repeat binding factor 2 antibody
    • TTAGGG repeat-binding factor 2 antibody
    see all

Images

  • Anti-TRF2 antibody - N-terminal (ab191704) at 1 µg/ml + 293 cell lysate at 15 µg

    Predicted band size: 59 kDa

  • Immunohistochemical analysis of human kidney tissue labeling TRF2 using ab191704 at 5 μg/ml.

References

ab191704 has not yet been referenced specifically in any publications.

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