Overview

  • Product name

  • Description

    Mouse polyclonal to TRFP/MED20
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human TRFP/MED20.

  • Positive control

    • TRFP/MED20 transfected 293T cell lysate and Hela cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance

    TRFP / MED20 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
  • Cellular localization

    Nucleus
  • Database links

  • Alternative names

    • MED20 antibody
    • Mediator complex subunit 20 antibody
    • TRF proximal protein homolog antibody
    • TRFP antibody

Images

  • All lanes : Anti-TRFP/MED20 antibody (ab67800) at 1/500 dilution

    Lane 1 : TRFP/MED20 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 23 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?

  • ab67800 at 10µg/ml staining TRFP/MED20 in Hela cells.

References

ab67800 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab67800.
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