Key features and details
- Mouse polyclonal to TRFP/MED20
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRFP/MED20 antibody
DescriptionMouse polyclonal to TRFP/MED20
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human TRFP/MED20.
- TRFP/MED20 transfected 293T cell lysate and Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
RelevanceTRFP / MED20 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
- MED20 antibody
- Mediator complex subunit 20 antibody
- TRF proximal protein homolog antibody
- TRFP antibody
All lanes : Anti-TRFP/MED20 antibody (ab67800) at 1/500 dilution
Lane 1 : TRFP/MED20 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
ab67800 at 10µg/ml staining TRFP/MED20 in Hela cells.
ab67800 has not yet been referenced specifically in any publications.