Key features and details
- Rabbit polyclonal to TRH-R
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRH-R antibody
See all TRH-R primary antibodies
DescriptionRabbit polyclonal to TRH-R
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human TRH-R aa 287-383.
- 293T, A431 and Raji cell lysates; Human OVCA xenograft tissue; A431 cells.
Previously labelled as TRH Receptor.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 45 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionReceptor for thyrotropin-releasing hormone. This receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- Thyroliberin receptor antibody
- Thyrotropin Releasing Hormone Receptor antibody
- Thyrotropin-releasing hormone receptor antibody
Immunofluorescent analysis of methanol-fixed A431 cells labeling TRH-R with ab151510 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Anti-TRH-R antibody (ab151510) at 1/2000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 45 kDa
Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue labeling TRH-R with ab151510 at 1/00 dilution.
ab151510 has been referenced in 1 publication.
- Dubovy SR et al. Expression of hypothalamic neurohormones and their receptors in the human eye. Oncotarget 8:66796-66814 (2017). PubMed: 28977997