Overview

  • Product name

  • Description

    Rabbit polyclonal to TRH-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TRH-R aa 287-383.

  • Positive control

    • 293T, A431 and Raji cell lysates; Human OVCA xenograft tissue; A431 cells.
  • General notes

    Previously labelled as TRH Receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab151510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Receptor for thyrotropin-releasing hormone. This receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Thyroliberin receptor antibody
    • Thyrotropin Releasing Hormone Receptor antibody
    • Thyrotropin-releasing hormone receptor antibody
    • TRFR_HUMAN antibody
    • TRH Receptor antibody
    • TRH-R antibody
    • TRHR antibody
    see all

Images

  • Immunofluorescent analysis of methanol-fixed A431 cells labeling TRH-R with ab151510 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Anti-TRH-R antibody (ab151510) at 1/2000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS-PAGE.
  • Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue labeling TRH-R with ab151510 at 1/00 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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