Overview

  • Product name

  • Description

    Rabbit polyclonal to TRH-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Dog, Human, Chimpanzee, Monkey
    Predicted to work with: Mouse, Rat, Cow, Opossum
  • Immunogen

    Synthetic peptide corresponding to Human TRH-R aa 350-400 (C terminal).

  • Positive control

    • Human brain lysate. Human thyroid tissue.
  • General notes

    Previously labelled as TRH Receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab72179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

Images

  • All lanes : Anti-TRH-R antibody (ab72179) at 0.5 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate with immunising peptide

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • ab72179 at 10µg/ml staining TRH-R in human thyroid by Immunohistochemistry using paraffin-embedded tissue.

References

This product has been referenced in:

  • Zhang Y  et al. The potential role of thyrotropin-releasing hormone in colonic dysmotility induced by water avoidance stress in rats. Neuropeptides N/A:N/A (2018). WB, IHC-P . Read more (PubMed: 29803395) »
  • Puissant MM  et al. RNASeq-derived transcriptome comparisons reveal neuromodulatory deficiency in the CO2 insensitive brown Norway rat. J Physiol 593:415-30 (2015). Read more (PubMed: 25630262) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Olfactory Bulb)
Permeabilization
Yes - 1% triton, 30 minutes
Specification
Olfactory Bulb
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 30 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 18 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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