Overview

  • Product name
    Anti-TRH Receptor antibody
    See all TRH Receptor primary antibodies
  • Description
    Rabbit polyclonal to TRH Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human, Chimpanzee, Monkey
    Predicted to work with: Mouse, Rat, Cow, Opossum
  • Immunogen

    Synthetic peptide corresponding to a portion of amino acids 350-400 of Human TRH Receptor

  • Positive control
    • Human brain lysate. Human thyroid tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab72179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

Images

  • All lanes : Anti-TRH Receptor antibody (ab72179) at 0.5 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate with immunising peptide

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • ab72179 at 10µg/ml staining TRH Receptor in human thyroid by Immunohistochemistry using paraffin-embedded tissue.

References

This product has been referenced in:
  • Zhang Y  et al. The potential role of thyrotropin-releasing hormone in colonic dysmotility induced by water avoidance stress in rats. Neuropeptides N/A:N/A (2018). WB, IHC-P . Read more (PubMed: 29803395) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Olfactory Bulb)
Permeabilization
Yes - 1% triton, 30 minutes
Specification
Olfactory Bulb
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 30 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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